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Detection Image Detection method details

Name:

 QL-ELE-00-012

Description:

 Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).

Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)

Comment:

 Target is the 35S promoter (P-35S) from Cauliflower mosaic virus. For details of the detection of nopaline synthase terminator (partim T-nos) by this duplex real-time PCR method refer to QL-ELE-00-013

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-ELE-00-013

Type:

 element-specific

Target DNA element:
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-FTM

  • Sequence:

    GCCTCTGCCGACAGTGGT

  • Size:

    18

  • Reverse Primer

  • Name:

    35S-RTM

  • Sequence:

    AAGACGTGGTTGGAACGTCTTC

  • Size:

    22

  • Probe

  • Name:

    35S-TMP-FAM

  • Sequence:

    CAAAGATGGACCCCCACCCACG

  • Size:

    22

  • Amplicon:

  • Sequence:

    gcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtctt

  • Size:

    82

Documents

Citation Type Local copy
Waiblinger H.-U., Ernst B., Anderson A., Pietsch K.; Validation and collaborative study of a P35S and T-nos duplex real-time PCR screening method to detect genetically modified organisms in food products Eur. Food Res. Technol. 226:1221-1228 (2008)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-012Link to the document url. EU reference method protocol