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Detection Image Detection method details

Name:

 QT-TAX-SL-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1)

Comment:

 target is the LAT52 gene from tomato (Solanum lycopersicum)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Solanum lycopersicum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    LAT52 primer F

  • Sequence:

    AGACCACGAGAACGATATTTGC

  • Size:

    22

  • Reverse Primer

  • Name:

    LAT52 primer R

  • Sequence:

    TTCTTGCCTTTTCATATCCAGACA

  • Size:

    24

  • Probe

  • Name:

    LAT52 probe

  • Sequence:

    CTCTTTGCAGTCCTCCCTTGGGCT

  • Size:

    24

  • Amplicon:

  • Sequence:

    agaccacgagaacgatatttgcnnnnnnnnnnnnnnnnnnagcccaagggaggactgcaaagagnnnntgtctggatatgaaaaggcaagaa

  • Size:

    92

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-SL-002Link to the document url. EU reference method protocol
Yang L., Zhang H., Guo J., Pan L., Zhang D.; International Collaborative Study of the Endogenous Reference Gene LAT52 Used for Qualitative and Quantitative Analyses of Genetically Modified Tomato J. Agric. Food Chem. 56:3438-3443 (2008)Link to the document url. peer-reviewed article