Help icon

Detection Image Detection method details

Name:

 QT-EVE-BN-011

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019).

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event Ms11 and the oilseed rape host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 Ms11

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SHA086

  • Sequence:

    CAAGATGGGAATTAACATCTACAAATTG

  • Size:

    28

  • Reverse Primer

  • Name:

    MDB371

  • Sequence:

    GAAATCCATGTAAAGCAGCAGGG

  • Size:

    23

  • Probe

  • Name:

    TM280

  • Sequence:

    CGACCATGTACATCCTACCA

  • Size:

    20

  • Amplicon:

  • Sequence:

    caagatgggaattaacatctacaaattgnnnnnnnnnnncgaccatgtacatcctaccannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccctgctgctttacatggatttc

  • Size:

    124

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-011Link to the document url. EU reference method protocol