Detection method details
Name: |
QT-TAX-ZM-006 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) |
Comment: |
Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
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Oligonucleotides:
Forward Primer
Name:
SSllb 1-5'Sequence:
CTCCCAATCCTTTGACATCTGCSize:
22
Reverse Primer
Name:
SSIIb 1-3'Sequence:
TCGATTTCTCTCTTGGTGACAGGSize:
23
Probe
Name:
SSIIb-TaqSequence:
AGCAAAGTCAGAGCGCTGCAATGCASize:
25
-
Amplicon:
Sequence:
ctcccaatcctttgacatctgcnnnnnagcaaagtcagagcgctgcaatgcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgtcaccaag agagaaatcgaSize:
151