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Detection Image Detection method details

Name:

 QT-EVE-ZM-012

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014)

Comment:

 Target is the integration border region (IBR) between the insert of maize event DAS59122 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 DAS59122

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DAS-59122-7-rb1f

  • Sequence:

    GGGATAAGCAAGTAAAAGCGCTC

  • Size:

    23

  • Reverse Primer

  • Name:

    DAS-59122-rb1r

  • Sequence:

    CCTTAATTCTCCGCTCATGATCAG

  • Size:

    24

  • Probe

  • Name:

    DAS-59122-7-rb1s

  • Sequence:

    TTTAAACTGAAGGCGGGAAACGACAA

  • Size:

    26

  • Amplicon:

  • Sequence:

    gggataagcaagtaaaagcgctcnnnnnnnnnnnntttaaactgaaggcgggaaacgacaanctgatcatgagcggagaattaagg

  • Size:

    86

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-012Link to the document url. EU reference method protocol