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Detection Image Detection method details

Name:

 QT-EVE-ZM-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570)

Comment:

 target is the 3' integration border region (IBR) between the insert of maize event Bt11 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 event-specific

Target GMO name:

 Bt11

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt113JFor

  • Sequence:

    GCGGAACCCCTATTTGTTTA

  • Size:

    20

  • Reverse Primer

  • Name:

    Bt113JRev

  • Sequence:

    TCCAAGAATCCCTCCATGAG

  • Size:

    20

  • Probe

  • Name:

    Bt113JFT

  • Sequence:

    AAATACATTCAAATATGTATCCGCTCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    gcggaacccctatttgtttannnnnnnaaatacattcaaatatgtatccgctcatggagggattcttgga

  • Size:

    70

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-006Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document