Detection method details
Name: |
QL-ELE-00-013 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
Comment: |
Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens
For details of the detection of CaMV 35S promoter by this duplex real-time PCR method refer to QL-ELE-00-012 |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
180-FSequence:
CATGTAATGCATGACGTTATTTATGSize:
25
Reverse Primer
Name:
180-RSequence:
TTGTTTTCTATCGCGTATTAAATGTSize:
25
Probe
Name:
TM-180YYSequence:
ATGGGTTTTTATGATTAGAGTCCCGCAASize:
28
-
Amplicon:
Sequence:
catgtaatgcatgacgttatttatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacatttaatacgcgatagaaaacaaSize:
84
Documents
Citation | Type | Local copy |
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