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Detection method details
Name: |
QL-ELE-00-010 |
Description: |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) |
Comment: |
Target is the Figwort mosaic virus 34S promoter (P-FMV) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
E-FMV, P-34S FMV
|
-
Oligonucleotides:
Forward Primer
Name:
FMV-1Sequence:
AAGCCTCAACAAGGTCAGSize:
18
Reverse Primer
Name:
FMV-2Sequence:
CTGCTCGATGTTGACAAGSize:
18
-
Amplicon:
Sequence:
aagcctcaacaaggtcagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncttgtcaacatcgagcagSize:
196
Documents
Citation | Type | Local copy |
---|---|---|
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007)![]() |
peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-ELE-00-010![]() |
EU reference method protocol |