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Detection Image Detection method details

Name:

 QL-ELE-cry3A-F/cry3A-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016)

Comment:

 target is the cry3A gene from bacterium Bacillus thuringiensis subsp. kumamotoensis (amplicon sequence taken from GenBank DI157749.1; MIR604 maize).

Validation:

 in-house validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-mcry3A-SYNTH, CS-cry3A-BACTT

  • Oligonucleotides:

  • Forward Primer

  • Name:

    cry3A-F

  • Sequence:

    cagcaacatcgagaacta

  • Size:

    18

  • Reverse Primer

  • Name:

    cry3A-R

  • Sequence:

    ctccagtagttgaagctg

  • Size:

    18

  • Probe

  • Name:

    cry3A-P

  • Sequence:

    tgttcgactacctgcaccgc

  • Size:

    20

  • Amplicon:

  • Sequence:

    cagcaacatcgagaactacatccgcaagccccacctgttcgactacctgcaccgcatccagttccacacgcgtttccagcccggctactacggcaacgacagcttcaactactggag

  • Size:

    117

Documents

Citation Type Local copy
Prins, T.W., van Hoof, R.A., Scholtens, I.M.J., Kok, E.J. (2016). Novel TaqMan PCR screening methods for element cry3A and construct gat/T‑pinII to support detection of both known and unknown GMOs. Eur Food Res Technol, 243, 481–488.Link to the document url. peer-reviewed article Download PDF pdf