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Detection Image Detection method details

Name:

 QL-ELE-RERIO 2206/RERIO 2207

Description:

 Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007).

Comment:

 Target is the modified red fluorescent protein (DsRed2) gene from Discosoma sp. (sequence in pDsRed2 vector of Clontech, see GenBank EA057759.1).

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-DsRed2 fluorescent protein

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RERIO 2206

  • Sequence:

    ACAACACCGTGAAGCTGAAGGTGACCAAG

  • Size:

    29

  • Reverse Primer

  • Name:

    RERIO 2207

  • Sequence:

    GGTGTAGTCCTCGTTGTGGGAGGTGATGTC

  • Size:

    30

  • Amplicon:

  • Sequence:

    acaacaccgtgaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttccagtacggctccaaggtgtacgtgaagcaccccgccgacatccccgactacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggcgaccgtgacccaggactcctccctgcaggacggctgcttcatctacaaggtgaagttcatcggcgtgaacttcccctccgacggccccgtgatgcagaagaagaccatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagacccacaaggccctgaagctgaaggacggcggccactacctggtggagttcaagtccatctacatggccaagaagcccgtgcagctgcccggctactactacgtggacgccaagctggacatcacctcccacaacgaggactacacc

  • Size:

    506

Documents

Citation Type Local copy
H. Rehbein & J. Bogerd (2007) Identification of Genetically Modified Zebrafish (Danio rerio) by Protein- and DNA-Analysis. J. Verbr. Lebensm. 2:122-125Link to the document url. peer-reviewed article