Detection method details
Name: |
QT-EVE-ZM-027 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018). |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event MZHG0JG and the maize host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
MZHG0JG |
-
Oligonucleotides:
Forward Primer
Name:
MZHG0JG forward primerSequence:
CAACTAGCTAGATTAATTAACGCAATCTGSize:
29
Reverse Primer
Name:
MZHG0JG reverse primerSequence:
ATTTGTTTGCAAGGTGTGGGASize:
21
Probe
Name:
MZHG0JG probeSequence:
TTAAGTTGTCTAAGCGTCAATTTGSize:
24
-
Amplicon:
Sequence:
caactagctagattaattaacgcaatctgnnnttaagttgtctaagcgtcaatttgnnnntcccacaccttgcaaacaaatSize:
81
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-027 | EU reference method protocol |