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Detection Image Detection method details

Name:

 QL-CON-00-005

Description:

 Qualitative conventional PCR method for detection of maize event T25 (ISO 21569)

Comment:

 Target is the junction region between the phosphinothricin N-acetyltransferase (CS-pat-STRVR) gene from Streptomyces viridochromogenes and the Cauliflower mosaic virus 35S terminator (T-35S-CaMV)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-TAX-ZM-003

Type:

 construct-specific

Target GMO names:
40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP32316, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T25-F7

  • Sequence:

    ATGGTGGATGGCATGATGTTG

  • Size:

    21

  • Reverse Primer

  • Name:

    T25-R3

  • Sequence:

    TGAGCGAAACCCTATAAGAACCC

  • Size:

    23

  • Amplicon:

  • Sequence:

    atggtggatggcatgatgttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngggttcttatagggtttcgctca

  • Size:

    209

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-005Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article