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Detection Image Detection method details

Name:

 QL-CON-CP1/pFMV2

Description:

 Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11).

Comment:

 Target is the junction region between the Figwort mosaic virus promoter (P-FMV) and the epsps gene from Agrobacterium tumefaciens strain CP4 (cp4-epsps).

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 construct-specific

Target GMO names:
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CP1

  • Sequence:

    GACTTACGAGCAGTTGCTGGACGGCTGC

  • Size:

    28

  • Reverse Primer

  • Name:

    pFMV2

  • Sequence:

    CCTGACAGCCCACTCACTAATGCGTATG

  • Size:

    28

  • Amplicon:

  • Sequence:

    cctgacagcccactcactaatgcgtatgacgaacgcagtgacgaccacaaaagaattccctctatataagaaggcattcattcccatttgaaggatcatcagatactcaaccaatccttctagaagatctaagcttatcgataagcttgatgtaattggaggaagatcaaaattttcaatccccattcttcgattgcttcaattgaagtttctccgatggcgcaagttagcagaatctgcaatggtgtgcagaacccatctcttatctccaatctctcgaaatccagtcaacgcaaatctcccttatcggtttctctgaagacgcagcagcatccacgagcttatccgatttcgtcgtcgtggggattgaagaagagtgggatgacgttaattggctctgagcttcgtcctcttaaggtcatgtcttctgtttccacggcgtgcatgcttcacggtgcaagcagccgtccagcaactgctcgtaagtc

  • Size:

    488

Documents

Citation Type Local copy
PCR detection of the pFMV-CP4EPSPS construct for screening of genetically modified plants - construct-specific method. BVL Official Method Collection G 30.40-11 Online Publication (2015)Link to the document url. other document