Detection method details
Name: |
QL-CON-gat/T-pinII-F/gat/T-pinII-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) |
Comment: |
Target is the junction between between the Bacillus licheniformis glyphosate-N-acetyltransferase (CS-gat-
BACLI) gene and the proteinase
inhibitor II (T-pinII-SOLTU) terminator from Solanum tuberosum.
Amplicon represented by maize 98140 (GenBank KP784699) is 112 nt instead of the published 111 nt. |
Validation: |
in-house validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
61061, 73496, 98140, DP356043
|
-
Oligonucleotides:
Forward Primer
Name:
gat/T-pinII-FSequence:
GGACCTCACATCCTGATGTATAASize:
23
Reverse Primer
Name:
gat/T-pinII-RSequence:
GCATCCTTTTATTTCATACATTASize:
23
Probe
Name:
gat/T-pinII-PSequence:
CCATCTTCTGGATTGGCCAACTTAASize:
25
-
Amplicon:
Sequence:
GGACCTCACATCCTGATGTATAAGAGGATCACATAACTAGCTAGTCAGTTAACCTAGACTTGTCCATCTTCTGGATTGGCCAACTTAATTAATGTATGAAATAAAAGGATGCSize:
112