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Detection Image Detection method details

Name:

 QL-ELE-00-009

Description:

 Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)

Comment:

 Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    HA-nos118-f

  • Sequence:

    GCATGACGTTATTTATGAGATGGG

  • Size:

    24

  • Reverse Primer

  • Name:

    HA-nos118-r

  • Sequence:

    GACACCGCGCGCGATAATTTATCC

  • Size:

    24

  • Amplicon:

  • Sequence:

    gcatgacgttatttatgagatgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggataaattatcgcgcgcggtgtc

  • Size:

    118

Documents

Citation Type Local copy
Lipp M., Bluth A., Eyquem F., Kruse L., Schimmel H., Van den Eede G., Anklam E.; Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs Eur. Food Res. Technol. 212:49Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-009Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document