Help icon

Detection Image Detection method details

Name:

 QT-EVE-GH-001a

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event 281-24-236 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-016, QT-TAX-GH-021

Type:

 event-specific

Target GMO name:

 281-24-236

  • Oligonucleotides:

  • Forward Primer

  • Name:

    281-f1

  • Sequence:

    CTCATTGCTGATCCATGTAGATTTC

  • Size:

    25

  • Reverse Primer

  • Name:

    281-r2

  • Sequence:

    GGACAATGCTGGGCTTTGTG

  • Size:

    20

  • Probe

  • Name:

    281-s2

  • Sequence:

    TTGGGTTAATAAAGTCAGATTAGAGGGAGACAA

  • Size:

    33

  • Amplicon:

  • Sequence:

    -

  • Size:

    111

Documents

Citation Type Local copy
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-001aLink to the document url. EU reference method protocol