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Detection Image Detection method details

Name:

 QL-TAX-OS-003

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005)

Comment:

 target is the endogenous root-specific GOS9 gene of rice

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    org1

  • Sequence:

    TTAGCCTCCCGCTGCAGA

  • Size:

    18

  • Reverse Primer

  • Name:

    org2

  • Sequence:

    AGAGTCCACAAGTGCTCCCG

  • Size:

    20

  • Probe

  • Name:

    orgp

  • Sequence:

    CGGCAGTGTGGTTGGTTTCTTCGG

  • Size:

    24

  • Amplicon:

  • Sequence:

    ttagcctcccgctgcagancggcagtgtggttggtttcttcggnnnnncgggagcacttgtggactct

  • Size:

    68

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-003Link to the document url. EU reference method protocol