Detection method details
Name: |
QT-EVE-ST-001 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of potato event EH92-527-1 and the potato host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
EH92-527-1 |
-
Oligonucleotides:
Forward Primer
Name:
Event527-bf1Sequence:
GTGTCAAAACACAATTTACAGCASize:
23
Reverse Primer
Name:
St527-R1Sequence:
TCCCTTAATTCTCCGCTCATGASize:
22
Probe
Name:
St527-S2Sequence:
AGATTGTCGTTTCCCGCCTTCAGTTSize:
25
-
Amplicon:
Sequence:
gtgtcaaaacacaatttacagcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaactgaaggcgggaaacgacaatctnntcatgagcggagaattaagggaSize:
134
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ST-001 | EU reference method protocol |