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Detection Image Detection method details

Name:

 QT-EVE-GH-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event MON1445 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-015

Type:

 event-specific

Target GMO name:

 MON1445

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON1445 primer forward

  • Sequence:

    GGAGTAAGACGATTCAGATCAAACAC

  • Size:

    26

  • Reverse Primer

  • Name:

    MON1445 primer reverse

  • Sequence:

    ATCGACCTGCAGCCCAAGCT

  • Size:

    20

  • Probe

  • Name:

    MON1445 probe

  • Sequence:

    ATCAGATTGTCGTTTCCCGCCTTCAGTTT

  • Size:

    29

  • Amplicon:

  • Sequence:

    ggagtaagacgattcagatcaaacacnnnnnnnnnaaactgaaggcgggaaacgacaatctgatnnnagcttgggctgcaggtcgat

  • Size:

    87

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-003Link to the document url. EU reference method protocol