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Detection Image Detection method details

Name:

 QT-EVE-CP-001

Description:

 Quantitative real-time (TaqMan) PCR method for detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 13, 2016).

Comment:

 Target is the 5' integration border region (IBR) between the insert of papaya event Huanong No 1 and the papaya host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-CP-001

Type:

 event-specific

Target GMO name:

 Huanong No. 1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    qHN-F

  • Sequence:

    GACGAGTACAAGGAGACGCC

  • Size:

    20

  • Reverse Primer

  • Name:

    qHN-R

  • Sequence:

    GTTGTCACTGAAGCGGGAAG

  • Size:

    20

  • Probe

  • Name:

    qHN-P

  • Sequence:

    TGGCTGCTATTGGGCGAATCAACTAC

  • Size:

    26

  • Amplicon:

  • Sequence:

    gacgagtacaaggagacgccttttaatttgtttaaattaaataaaaacataaattattaagcgacgtaataataaacacgtcataaattttttcaaaaaataaaatttcttttattttctaaaaagtagttgattcgcccaatagcagccagtcccttcccgcttcagtgacaac

  • Size:

    175

Documents

Citation Type Local copy
Guo, J. C., Yang, L. T., Liu, X., Guan, X. Y., Jiang, L. X., & Zhang, D. B. (2009). Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong no. 1 papaya. Journal of Agricultural and Food Chemistry, 57(16), 7205-7212.Link to the document url. peer-reviewed article
Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-EVE-CP-001Link to the document url. EU reference method protocol