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Detection Image Detection method details

Name:

 QL-TAX-LU-001

Description:

 Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020)

Comment:

 Target is the endogenous stearoyl-acyl carrier protein desaturase gene (sad) of Linum usitatissimum

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 taxon-specific

Target Species name:

 Linum usitatissimum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SAD-FW

  • Sequence:

    GCTCAACCCAGTCACCACCT

  • Size:

    20

  • Reverse Primer

  • Name:

    SAD-RV

  • Sequence:

    TGCGAGGAGATCTGGAGGAG

  • Size:

    20

  • Probe

  • Name:

    SAD-probe

  • Sequence:

    TGTTGAGGGAGCGTGTTGAAGGGA

  • Size:

    24

  • Amplicon:

  • Sequence:

    gctcaaccca gtcaccacct tcccttcaac acgctccctc aacannnnct cctccagatc tcctcgca

  • Size:

    68

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-LU-001Link to the document url. EU reference method protocol