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Detection Image Detection method details

Name:

 QL-ELE-npt 1-5'/npt 1-3'

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).

Comment:

 Target is the neomycin phosphotransferase II (nptII) gene from bacterium Escherichia coli.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-nptII-ECOLX, V-nptII-ECOLX

  • Oligonucleotides:

  • Forward Primer

  • Name:

    npt 1-5'

  • Sequence:

    GACAGGTCGGTCTTGACAAAAAG

  • Size:

    23

  • Reverse Primer

  • Name:

    npt 1-3'

  • Sequence:

    GAACAAGATGGATTGCACGC

  • Size:

    20

  • Probe

  • Name:

    nptII-probe

  • Sequence:

    TGCCCAGTCATAGCCGAATAGCCTCTCCA

  • Size:

    29

  • Amplicon:

  • Sequence:

    gacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttc

  • Size:

    155

Documents

Citation Type Local copy
Matsuoka T, Kuribara H, Takubo K, Akiyama H, Miura H, Goda Y, Kusakabe Y, Isshiki K, Toyoda M, Hino A (2002) Detection of recombinant DNA segments introduced to genetically modified maize (*Zea mays*). J Agric Food Chem 50, 2100-2109Link to the document url.