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Detection Image Detection method details

Name:

 QL-CON-GH-tFreeze-F/GH-tFreeze-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182).

Comment:

 Target is the junction region between the growth hormone gene (CS-GHc2) sequence from Oncorhynchus tshawytscha and the antifreeze terminator (T-AFP) from Zoarces americanus.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 construct-specific

Target GMO names:
AquAdvantage Salmon

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GH-tFreeze-F

  • Sequence:

    CTCCACAGGTTTTGACATGTTCA

  • Size:

    23

  • Reverse Primer

  • Name:

    GH-tFreeze-R

  • Sequence:

    GCCAGCAAGAGCCCATCTC

  • Size:

    19

  • Probe

  • Name:

    GH-tFreeze-P

  • Sequence:

    TTCCTAATCTGTATCTGGGAAACCGAACCCT

  • Size:

    31

  • Amplicon:

  • Sequence:

    ctccacaggttttgacatgttcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagatgggctcttgctggc

  • Size:

    75

Documents

Citation Type Local copy
Protocol QL-CON-GH-tFreeze-F/GH-tFreeze-R, Debode et al. 2017 PCR Protocol Download PDF pdf
Debode F., Janssen E., Marien A. et al. (2018) Detection of transgenic atlantic and Coho Salmon by real-time PCR. Food Analytical Methods,11(9),2396–2406Link to the document url. peer-reviewed article