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Detection Image Detection method details

Name:

 QT-ELE-NOS ter 2-5' /NOS ter 2-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)

Comment:

 target is the nopaline synthase terminator (T-nos) from bacterium Agrobacterium tumefaciens. Amplicon was generated from cotton LLcotton25 (GenBank HQ233646.1)

Validation:

 ring trial validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    NOS ter 2-5'

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTG

  • Size:

    29

  • Reverse Primer

  • Name:

    NOS ter 2-3'

  • Sequence:

    CGCTATATTTTGTTTTCTATCGCGT

  • Size:

    25

  • Probe

  • Name:

    NOS-Taq

  • Sequence:

    AGATGGGTTTTTATGATTAGAGTCCCGCAA

  • Size:

    30

  • Amplicon:

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCG

  • Size:

    151

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