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Detection Image Detection method details

Name:

QT-EVE-ZM-034

Description:

Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023)

Comment:

Target is the 5' integration border region (IBR) between the insert of maize event DP202216 and the maize host genome.

Validation:

ring trial validation

Standardisation:

EU reference method

Related Methods:

QT-TAX-ZM-008

Type:

event-specific

Target GMO name:

DP202216
  • Oligonucleotides:

  • Forward Primer

  • Name:

    PHN165665
  • Sequence:

    CCATCTGAGGTCTGCACTCTCAC
  • Size:

    23
  • Reverse Primer

  • Name:

    PHN143487
  • Sequence:

    CTCCGCTCATGATCAGATTGTC
  • Size:

    22
  • Probe

  • Name:

    PHN-2074 Probe 1
  • Sequence:

    CAACACACTCAAACACTGATAG
  • Size:

    22
  • Amplicon:

  • Sequence:

    ccatctgaggtctgcactctcacnnnnnnnnnnnnnnnnncaacacactcaaacactgatagnnnnnnnnnnnnnnnnnnnnngacaatctgatcatgagcggag
  • Size:

    105

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-034Link to the document url. EU reference method protocol