Detection method details
Name: |
QT-EVE-GM-007 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of soybean event A5547-127 and the soybean host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GM-020 |
Type: |
event-specific |
Target GMO name: |
A5547-127 |
-
Oligonucleotides:
Forward Primer
Name:
SHA003Sequence:
GCTATTTGGTGGCATTTTTCCASize:
22
Reverse Primer
Name:
SHA004Sequence:
CACTGCGGCCAACTTACTTCTSize:
21
Probe
Name:
TM058Sequence:
CCGCAATGTCATACCGTCATCGTTGTSize:
26
-
Amplicon:
Sequence:
gctatttggtggcatttttccannnnnccgcaatgtcataccgtcatcgttgtnagaagtaagttggccgcagtgSize:
75
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-007 | EU reference method protocol | |
Delobel C., Bogni A., Mazzara M., Van den Eede G.; Event-specific Method for the Quantification of Soybean Line A5547-127 Using Real-time PCR - Validation Report and Protocol Online Publication (2009) | EU reference method protocol |