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Detection Image Detection method details

Name:

 QT-CON-00-008

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002)

Comment:

 Target is the junction region between an optimized transit peptide sequence (OTP) and point mutated epsps gene (mEPSPS) from maize

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-ZM-006

Type:

 construct-specific

Target GMO names:
DAS44406, FG72, GA21, GHB614, GHB811

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GA21 3-5'

  • Sequence:

    GAAGCCTCGGCAACGTCA

  • Size:

    18

  • Reverse Primer

  • Name:

    GA21 3-3'

  • Sequence:

    ATCCGGTTGGAAAGCGACTT

  • Size:

    20

  • Probe

  • Name:

    GA21-2-Taq

  • Sequence:

    AAGGATCCGGTGCATGGCCG

  • Size:

    20

  • Amplicon:

  • Sequence:

    gaagcctcggcaacgtcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaagtcgctttccaaccggat

  • Size:

    133

Documents

Citation Type Local copy
Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J., Akiyama H., Goda Y., Toyoda M., Hino A.; Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules J AOALink to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-CON-00-008Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document