Detection method details
Name: |
QL-EVE-Kef6 forward/Kef6 reverse |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) |
Comment: |
Target is the 3' junction between the insert of rice event Kefeng6 and the rice host genome |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
Kefeng6 |
-
Oligonucleotides:
Forward Primer
Name:
Kef6 forwardSequence:
TGGATCAGATTGTCGTTTCCCGCCTTSize:
26
Reverse Primer
Name:
Kef6 reverseSequence:
CAAGAAGCAAGCTGAGGCAAACAAGCTSize:
27
Probe
Name:
Kef6 hydrolysis probeSequence:
AGGGCGATTGCTGGCGAGGCSize:
20
-
Amplicon:
Sequence:
tggatcagattgtcgtttcccgccttcagtttaaactatcagcgacaaaagatcaggatttgggaagggcgattgctggcgaggcacatatagctccatatagcttgtttgcctcagcttgcttcttgSize:
128