Detection Image Detection methods search result

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QL-CON-00-013 construct-specific
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015) ring trial validation EU reference method
QL-CON-307-F/652-R construct-specific
KMD1, Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013) in-house validation none
QL-CON-35S-F/nptII-R construct-specific
23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON863, MON87460, SPBT02-7, ZW20
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010) in-house validation none
QL-CON-35S-5' primer A/A1-3' primer B construct-specific
RL01-15, RL01-17, dfr GM petunia
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) unknown none
QL-ELE-AINT 2-5'/AINT 2-3' element-specific
I-actin
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). in-house validation none
QL-ELE-At-nop-f2/At-nop-r2 element-specific
CS-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the nopaline synthase gene (nos) from Agrobacterium tumefaciens (BVL G30.40-16) ring trial validation national standard
QL-ELE-bar 2-5'/bar 2-3' element-specific
CS-bar-STRHY
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) in-house validation none
QL-ELE-B'nase-F-n4/BNaseR-n3 element-specific
CS-barnase
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013) in-house validation none
QL-ELE-BstarF-n2 /BstarR-n2 element-specific
CS-barstar
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) in-house validation none
QL-ELE-cat-F/R element-specific
V-chloramphenicol acetyl transferase
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).

In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2).

unknown none
QL-ELE-cat-F1/R1 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2.

unknown none
QL-ELE-cat-F2/cat-R2 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method.

unknown none
QL-ELE-cry1A 4-5'/cry1A 4-3' element-specific
CS-cry1Ab-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013) in-house validation none
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 element-specific
CS-cry1Ac-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013) in-house validation none
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 element-specific
CS-cry1A_105-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011) in-house validation none
QL-ELE-Cry1F-F2/Cry1Fr-n2 element-specific
CS-cry1F-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) in-house validation none
QL-ELE-cry2Ab2 - F/cry2Ab2 - R element-specific
CS-cry2Ab2-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011) in-house validation none
QL-ELE-cry3A-F/cry3A-R element-specific
CS-cry3A-BACTU, CS-mcry3A-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) in-house validation national standard
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 element-specific
CS-cry3Bb1-BACTU
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD) in-house validation none
QL-ELE-epsps 1-5'/epsps 3-3' element-specific
CS-CP4epsps-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013) in-house validation none
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