Detection Image Detection methods search result

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QL-CON-35S-5' primer A/A1-3' primer B construct-specific
RL01-15, RL01-17, dfr GM petunia
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) unknown none
QL-ELE-cat-F1/R1 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2.

unknown none
QL-ELE-cat-F/R element-specific
V-chloramphenicol acetyl transferase
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).

In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2).

unknown none
QL-ELE-cat-F2/cat-R2 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method.

unknown none
QL-EVE-558F/558R event-specific
e871 Bacillus subtilis
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017) unknown none
QL-ELE-RERIO 2206/RERIO 2207 element-specific
CS-DsRed2 fluorescent protein
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007) unknown none
QL-EVE-16-0-1RB-F/16-0-1RB-R event-specific
16-0-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) unknown none
QL-EVE-18-2-4RB-F/18-2-4RB-R event-specific
18-2-4
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) unknown none
QT-EVE-GH-006 event-specific
GHB614
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-CON-00-002 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS-40-3-2 (Hird et al., 2003). ring trial validation national standard
QT-TAX-GM-007 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-CON-00-001 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS-40-3-2 (ISO 21570) ring trial validation ISO standard
QT-EVE-ST-001 event-specific
EH92-527-1
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) ring trial validation EU reference method
QT-TAX-GH-019 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-EVE-GH-007 event-specific
MON88913
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) ring trial validation EU reference method
QT-EVE-BN-001 event-specific
T45
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-TAX-BN-012 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-EVE-BN-002 event-specific
Ms8
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-003 event-specific
Rf3
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-004 event-specific
GT73
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
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