Detection methods search result
Your search criterion is: ( method set: ALL )
Refine Search
Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | RL01-15, RL01-17, dfr GM petunia |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
| QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007) | unknown | none |
| QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |
| QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017) | unknown | none |
| QT-EVE-ZM-003 | event-specific | MON87427 |
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). | ring trial validation | EU reference method |
| QT-EVE-BV-001 | event-specific | H7-1 |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-013 | event-specific | MIR604 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-001 | event-specific | FG72 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-010 | event-specific | MON88701 |
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) | ring trial validation | EU reference method |
| QL-ELE-00-016 | element-specific | CS-cry1Ab-BACTU, CS-cry1Ab_Ac-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6) | ring trial validation | ISO standard |
| QT-EVE-ZM-009 | event-specific | MON863 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
| QT-CON-00-006 | construct-specific | Bt10 maize, Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-EVE-BN-010 | event-specific | MON88302 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-ELE-At-nop-f2/At-nop-r2 | element-specific | CS-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the nopaline synthase gene (nos) from Agrobacterium tumefaciens (BVL G30.40-16) | ring trial validation | national standard |
| QL-TAX-CaMV-ORFV-fd2/CaMV-ORFV-rd2 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)(BVL G30.40-17) | ring trial validation | national standard |