Detection Image Detection methods search result

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QL-CON-35S-5' primer A/A1-3' primer B construct-specific
RL01-15, RL01-17, dfr GM petunia
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) unknown none
QL-ELE-RERIO 2206/RERIO 2207 element-specific
CS-DsRed2 fluorescent protein
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007) unknown none
QL-EVE-16-0-1RB-F/16-0-1RB-R event-specific
16-0-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) unknown none
QL-EVE-18-2-4RB-F/18-2-4RB-R event-specific
18-2-4
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) unknown none
QL-ELE-cat-F1/R1 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2.

unknown none
QL-ELE-cat-F/R element-specific
V-chloramphenicol acetyl transferase
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).

In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2).

unknown none
QL-ELE-cat-F2/cat-R2 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method.

unknown none
QL-EVE-558F/558R event-specific
e871 Bacillus subtilis
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017) unknown none
QT-EVE-ZM-003 event-specific
MON87427
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). ring trial validation EU reference method
QT-EVE-BV-001 event-specific
H7-1
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-013 event-specific
MIR604
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-001 event-specific
FG72
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GH-010 event-specific
MON88701
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) ring trial validation EU reference method
QL-ELE-00-016 element-specific
CS-cry1Ab-BACTU, CS-cry1Ab_Ac-SYNTH, CS-cry1Ac-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6) ring trial validation ISO standard
QT-EVE-ZM-009 event-specific
MON863
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-CON-00-006 construct-specific
Bt10 maize, Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) ring trial validation ISO standard
QT-EVE-BN-010 event-specific
MON88302
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QL-PLN-00-007 taxon-specific
all-plants
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) ring trial validation ISO standard
QL-ELE-At-nop-f2/At-nop-r2 element-specific
CS-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the nopaline synthase gene (nos) from Agrobacterium tumefaciens (BVL G30.40-16) ring trial validation national standard
QL-TAX-CaMV-ORFV-fd2/CaMV-ORFV-rd2 taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)(BVL G30.40-17) ring trial validation national standard
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