Detection Image Detection methods search result

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QL-BAC-27f/926R taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QT-TAX-GM-002 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QL-TAX-CaMVF/CaMVR taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) in-house validation none
QT-TAX-SPS primer f/SPS primer r taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) ring trial validation ISO standard
QL-TAX-GM-008 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) ring trial validation ISO standard
QL-TAX-OS-002 taxon-specific
Oryza sativa
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). in-house validation unknown
QT-TAX-ZM-014 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-ZM-011 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) ring trial validation EU reference method
QT-TAX-ZM-002 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-OS-017 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QL-PLN-00-007 taxon-specific
all-plants
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) ring trial validation ISO standard
QL-TAX-CaMV-ORFV-fd2/CaMV-ORFV-rd2 taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)(BVL G30.40-17) ring trial validation national standard
QL-TAX-DC-001 taxon-specific
Dianthus caryophyllus
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). in-house validation EU reference method
QL-TAX-FMVorf7-F4/FMVorf7-R4 taxon-specific
Figwort mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012) in-house validation national standard
QL-TAX-SL-001 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). ring trial validation ISO standard
QL-TAX-GM-002 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). ring trial validation ISO standard
QT-TAX-OS-003 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) ring trial validation unknown
QT-TAX-SL-002 taxon-specific
Solanum lycopersicum
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) ring trial validation ISO standard
QT-TAX-ZM-001 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 (adh1) gene.

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QL-BAC-16S-FW/16S-Rev taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
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