Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QT-TAX-CP-001 | taxon-specific | Carica papaya |
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) | ring trial validation | EU reference method |
| QT-TAX-OS-002 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
| QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-BN-003 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) | ring trial validation | EU reference method |
| QT-TAX-GM-003 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QL-TAX-Acc1-F/Acc1-R | taxon-specific | Medicago sativa |
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018) | ring trial validation | national standard |
| QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
| QT-TAX-ZM-004 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
| QT-TAX-ZM-005 | taxon-specific | Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). | ring trial validation | EU reference method |
| QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012) | in-house validation | national standard |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-TAX-CaMV-ORFV-fd2/CaMV-ORFV-rd2 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)(BVL G30.40-17) | ring trial validation | national standard |
| QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
| QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
| QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QT-TAX-OS-003 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) | ring trial validation | unknown |
| QT-TAX-SL-002 | taxon-specific | Solanum lycopersicum |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) | ring trial validation | ISO standard |
| QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcoholdeydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |