Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QT-EVE-GH-003 | event-specific | MON1445 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-TAX-GH-015 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
| QT-EVE-GH-004 | event-specific | MON531 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-022 | event-specific | MIR162 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-005 | event-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-006 | event-specific | MON89788 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON89788 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-007 | event-specific | A5547-127 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-TAX-GM-020 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-GH-019 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-EVE-GH-007 | event-specific | MON88913 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-001 | event-specific | T45 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-TAX-BN-012 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
| QT-EVE-BN-002 | event-specific | Ms8 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-003 | event-specific | Rf3 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-004 | event-specific | GT73 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-006 | event-specific | GHB614 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ST-001 | event-specific | EH92-527-1 |
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-005 | event-specific | Ms1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-006 | event-specific | Rf1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-008 | event-specific | Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | in-house validation | EU reference method |
| QT-EVE-GM-011 | event-specific | CV127 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-008 | event-specific | NK603 |
Quantitative real-time PCR (TaqMan) method for detection of maize event NK603 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-TAX-BV-013 | taxon-specific | Beta vulgaris |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) | ring trial validation | EU reference method |
| QT-TAX-GM-009 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) | ring trial validation | EU reference method |
| QT-TAX-OS-017 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-TAX-ZM-011 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) | ring trial validation | EU reference method |
| QT-TAX-ZM-014 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QL-ELE-00-015 | element-specific | E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). | ring trial validation | ISO standard |
| QL-ELE-00-008 | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) | ring trial validation | ISO standard |
| QT-CON-00-003 | construct-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570). | ring trial validation | ISO standard |
| QL-EVE-ZM-001 | event-specific | MON810 |
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-CON-00-012 | construct-specific | 55-1, 63-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) | ring trial validation | ISO standard |
| QT-CON-00-004 | construct-specific | DBN9936, MON80100, MON802, MON809, MON810 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-ELE-00-003 | element-specific | CS-cry1Ab-BACTU |
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) | ring trial validation | ISO standard |
| QT-TAX-GM-001 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene | ring trial validation | ISO standard |
| QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QT-TAX-SL-002 | taxon-specific | Solanum lycopersicum |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) | ring trial validation | ISO standard |
| QL-EVE-BN-002 | event-specific | GT73 |
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). | ring trial validation | ISO standard |
| QL-CON-00-001 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-CON-00-003 | construct-specific | Bt10 maize, Bt11 |
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-002 | construct-specific | Nema282F |
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-TAX-CMV-001 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-TAX-AT-001 | element-specific | CS-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QT-CON-00-006 | construct-specific | Bt10 maize, Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
| QL-ELE-00-016 | element-specific | CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). | ring trial validation | ISO standard |
| QL-CON-00-010 | construct-specific | FP967 |
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) | ring trial validation | ISO standard |
| QT-TAX-GM-002 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QT-EVE-ZM-006 | event-specific | Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570) | ring trial validation | ISO standard |
| QT-EVE-ZM-020 | event-specific | MON810 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570) | ring trial validation | ISO standard |
| QL-CON-00-014 | construct-specific | 1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) | ring trial validation | ISO standard |
| QT-CON-00-001 | construct-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO 21570) | ring trial validation | ISO standard |
| QT-TAX-GM-007 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene | ring trial validation | ISO standard |
| QT-CON-00-007 | construct-specific | B827, Bt176 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-TAX-ZM-006 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) | ring trial validation | ISO standard |
| QT-CON-00-008 | construct-specific | DAS44406, FG72, GA21, GHB614, GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-CON-00-005 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-CON-00-011 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DHA, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3). | ring trial validation | ISO standard |
| QT-ELE-00-004 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) | ring trial validation | ISO standard |
| QL-CON-00-005 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-ZM-003 | taxon-specific | Zea mays |
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-006 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-007 | construct-specific | Huahui No.1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) | ring trial validation | ISO standard |
| QL-CON-00-008 | construct-specific | 2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). | ring trial validation | ISO standard |
| QL-ELE-00-002 | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-00-004 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) | ring trial validation | ISO standard |
| QL-ELE-00-005 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-ELE-00-009 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) | ring trial validation | ISO standard |
| QL-ELE-00-010 | element-specific | E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) | ring trial validation | ISO standard |
| QL-CON-00-004 | construct-specific | Bt176 |
Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-00-011 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). | ring trial validation | ISO standard |
| QL-ELE-00-012 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-00-013 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-00-014 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) | ring trial validation | ISO standard |
| QT-TAX-ZM-002 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QL-TAX-GM-008 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) | ring trial validation | ISO standard |
| QT-TAX-SPS primer f/SPS primer r | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) | ring trial validation | ISO standard |
| QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-CON-CsVMV F/patsyn R | construct-specific | DAS44406, DAS68416, DAS81419 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |
| QL-CON-CP1/pFMV2 | construct-specific | GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). | ring trial validation | national standard |
| QL-CON-PGS-bar-A2/PGS-bar-B2 | construct-specific | Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea) |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). | ring trial validation | national standard |
| QL-EVE-816AvaF/817AvaR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 1 – Avalon) (BVL G30.40-6). | ring trial validation | national standard |
| QL-EVE-319FalF/320FalR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 2 – Falcon) (BVL G30.40-6) | ring trial validation | national standard |
| QL-EVE-375LibF/376LibR | event-specific | Liberator |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6). | ring trial validation | national standard |
| QL-CON-gat/T-pinII-F/gat/T-pinII-R | construct-specific | 61061, 73496, 98140, DP356043 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) | in-house validation | national standard |
| QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QL-CON-GH-tFreeze-F/GH-tFreeze-R | construct-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). | ring trial validation | national standard |
| QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
| QL-TAX-Acc1-F/Acc1-R | taxon-specific | Medicago sativa |
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-KK179-F/KK179-R | event-specific | KK179 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-J163-F/J163-R | event-specific | J163 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-J101-F/J101-R | event-specific | J101 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018) | ring trial validation | national standard |
| QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
| QL-TAX-OS-003 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) | ring trial validation | national standard |
| QT-ELE-P35S 1-5'/P35S 1-3' | element-specific | P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) | ring trial validation | national standard |