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QL-ELE-RERIO 2206/RERIO 2207 element-specific
CS-DsRed2 fluorescent protein
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). unknown none
QL-EVE-18-2-4RB-F/18-2-4RB-R event-specific
18-2-4
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) unknown none
QL-EVE-16-0-1RB-F/16-0-1RB-R event-specific
16-0-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) unknown none
QL-ELE-cat-F2/cat-R2 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method.

unknown none
QL-ELE-cat-F/R element-specific
V-chloramphenicol acetyl transferase
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).

In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2).

unknown none
QL-ELE-cat-F1/R1 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2.

unknown none
QT-TAX-zSSIIb-3F/zSSIIb-4R taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) unknown unknown
QL-CON-35S-5' primer A/A1-3' primer B construct-specific
A1-DFR Petunias, RL01-15, RL01-17
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) unknown none
QL-EVE-558F/558R event-specific
e871 Bacillus subtilis
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). unknown none
QL-EVE-ZM-P3waxy/P2waxy event-specific
GE-CRISPR-Cas Waxy Corn
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). unknown none
QL-EVE-ZM-P1waxy/P2waxy event-specific
GE-CRISPR-Cas Waxy Corn
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). unknown none
QL-CON-35S-F/nptII-R construct-specific
23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). in-house validation none
QL-CON-307-F/652-R construct-specific
KMD1, Kefeng6
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013). in-house validation none
QL-TAX-LU-001 taxon-specific
Linum usitatissimum
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) in-house validation none
QL-ELE-cry3A-F/cry3A-R element-specific
CS-cry3A-BACTT, CS-mcry3A-SYNTH
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) in-house validation national standard
QL-ELE-T-g7-F/T-g7-R element-specific
T-orf23-RHIRD, T-transcript 7
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) in-house validation none
QL-ELE-T-OCS-F/T-OCS-R element-specific
T-ocs-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) in-house validation none
QL-ELE-P-Rice actin-F/P-Rice actin-R element-specific
P-act1-ORYSA
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). in-house validation none
QL-ELE-P-TA29-F/P-TA29-R element-specific
P-ta29-TOBAC
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) in-house validation none
QL-ELE-P-SSuAra-F/P-SSuAra-R element-specific
P-rbcS-ARATH
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) in-house validation none
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