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QL-ELE-00-009 element-specific
T-nos-RHIRD
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) ring trial validation ISO standard
QL-TAX-BN-004 taxon-specific
Brassica napus
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). ring trial validation unknown
QL-TAX-OS-006 taxon-specific
Oryza sativa
Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009). ring trial validation unknown
QL-CON-00-001 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) ring trial validation ISO standard
QL-CON-00-006 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) ring trial validation ISO standard
QL-TAX-GM-008 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) ring trial validation ISO standard
QL-TAX-GM-002 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). ring trial validation ISO standard
QL-TAX-CP-001 taxon-specific
Carica papaya
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) ring trial validation EU reference method
QL-ELE-cat-F2/cat-R2 element-specific
V-chloramphenicol acetyl transferase
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).

In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method.

unknown none
QL-CON-PGS-bar-A2/PGS-bar-B2 construct-specific
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). ring trial validation national standard
QL-CON-CP1/pFMV2 construct-specific
GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). ring trial validation national standard
QL-TAX-ZM-003 taxon-specific
Zea mays
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) ring trial validation ISO standard
QL-ELE-RERIO 2206/RERIO 2207 element-specific
CS-DsRed2 fluorescent protein
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). unknown none
QL-ELE-00-003 element-specific
CS-nptII-ECOLX, V-nptII-ECOLX
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) ring trial validation national standard
QL-TAX-SL-005 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). ring trial validation unknown
QL-CON-00-002 construct-specific
Nema282F
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) ring trial validation ISO standard
QL-TAX-SL-001 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). ring trial validation ISO standard
QL-EVE-DC-001 event-specific
123.2.38
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). in-house validation EU reference method
QL-EVE-DC-003 event-specific
25958
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) in-house validation EU reference method
QL-EVE-ZM-001 event-specific
MON810
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). ring trial validation ISO standard
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