Detection methods search result
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Name | Type | Target | Description | Validation | Standardisation |
---|---|---|---|---|---|
QT-TAX-ZM-014 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
QT-TAX-ZM-003 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-TAX-GH-015 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-EVE-GH-009 | event-specific | T304-40 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event T304-40 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-007 | event-specific | MON88913 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-004 | event-specific | MON531 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-003 | event-specific | MON1445 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-002 | event-specific | LLCotton25 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-013 | event-specific | GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB811 (EU-RL GMFF GMOMETHODS database as of August 5, 2020) | ring trial validation | EU reference method |
QT-EVE-GH-006 | event-specific | GHB614 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-008 | event-specific | GHB119 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB119 (EU-RL GMFF GMOMETHODS database as of July 21, 2014). | ring trial validation | EU reference method |
QT-EVE-GH-012 | event-specific | COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) | ring trial validation | EU reference method |
QT-EVE-GH-001b | event-specific | 3006-210-23 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 3006-210-23 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-001a | event-specific | 281-24-236 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-GH-018 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' | element-specific | T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) | ring trial validation | none |
QT-EVE-ZM-028 | event-specific | MZIR098 |
Quantitative real-time PCR (TaqMan) method for detection of MZIR098. (EURL GMFF GMOMETHODS database as of 2018). | ring trial validation | EU reference method |
QT-EVE-ZM-027 | event-specific | MZHG0JG |
Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018). | ring trial validation | EU reference method |
QT-ELE-00-004 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) | ring trial validation | ISO standard |
QT-ELE-00-001 | element-specific | P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) | ring trial validation | unknown |
QT-EVE-OS-001 | event-specific | Golden Rice 2 |
Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
QT-TAX-GM-005 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
QT-EVE-BN-014 | event-specific | MON94100 |
Quantitative real-time PCR (TaqMan) for detection of oilseed rape event MON94100 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
QT-TAX-ZM-004 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-EVE-HF-1F/HF-2R | event-specific | Huafan No 1 |
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) | in-house validation | unknown |
QT-EVE-CP-001 | event-specific | Huanong No. 1 |
Quantitative real-time (TaqMan) PCR method for detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 13, 2016). | ring trial validation | EU reference method |
QT-TAX-CP-001 | taxon-specific | Carica papaya |
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) | ring trial validation | EU reference method |
QT-EVE-GM-016 | event-specific | MON87751 |
Quantitative PCR method for detection of soybean event MON87751 (EURL GMFF GMOMETHODS database as of April 21, 2017) | ring trial validation | EU reference method |
QT-EVE-GM-014 | event-specific | DAS81419 |
Quantitative PCR method for detection of soybean event DAS81419 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
QT-EVE-GM-015 | event-specific | DAS44406 |
Quantitative PCR method for detection of soybean event DAS44406 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
QT-TAX-GH-016 | taxon-specific | Gossypium hirsutum |
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-TAX-ZM-005 | taxon-specific | Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). | ring trial validation | EU reference method |
QT-EVE-ZM-030 | event-specific | MON87429 |
Quantitative PCR method for detection of maize event MON87429 (EU-RL GMFF GMOMETHODS database as of August 30, 2021) | ring trial validation | EU reference method |
QT-EVE-ZM-003 | event-specific | MON87427 |
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). | ring trial validation | EU reference method |
QT-EVE-ZM-024 | event-specific | MON87411 |
Quantitative PCR method for detection of maize event MON87411 (EURL GMFF GMOMETHODS database, as of April 21, 2017) | ring trial validation | EU reference method |
QT-EVE-ZM-025 | event-specific | MON87403 |
Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018) | ring trial validation | EU reference method |
QT-EVE-ZM-031 | event-specific | DP915635 |
Quantitative PCR method for detection of maize event DP915635 (EU-RL GMFF GMOMETHODS database as of May 5, 2023). | ring trial validation | EU reference method |
QT-EVE-ZM-026 | event-specific | 4114 |
Quantitative PCR method for detection of maize event 4114 (EURL GMFF GMOMETHODS database, as of May 24, 2018). | ring trial validation | EU reference method |
QT-EVE-GH-010 | event-specific | MON88701 |
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) | ring trial validation | EU reference method |
QT-EVE-GH-011 | event-specific | DAS81910 |
Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019) | ring trial validation | EU reference method |
QL-TAX-Acc1-F/Acc1-R | taxon-specific | Medicago sativa |
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018). | ring trial validation | national standard |
QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
QL-EVE-ZM-P1waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
QL-EVE-ZM-P3waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
QL-EVE-EC-001 | event-specific | PT73 E. coli (TM) |
Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017) | ring trial validation | EU reference method |
QL-EVE-EC-002 | event-specific | PL73 E. coli (LM) |
Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017) | ring trial validation | EU reference method |
QL-EVE-SQ0718/SQ0719 | event-specific | MON71800 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013). | in-house validation | none |
QL-EVE-TA-MON71200 | event-specific | MON71200 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). | in-house validation | EU reference method |
QL-ELE-P-Ubi-F/P-Ubi-R | element-specific | P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) | in-house validation | none |
QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
QL-ELE-00-024 | element-specific | T-rbcS_E9-PEA |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). | ring trial validation | EU reference method |
QL-ELE-00-025 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. | ring trial validation | EU reference method |
QL-TAX-PS-001 | taxon-specific | Pisum sativum |
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) | ring trial validation | EU reference method |
QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none |
QL-ELE-npt 1-5'/npt 1-3' | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). | in-house validation | none |
QL-CON-00-014 | construct-specific | 1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) | ring trial validation | ISO standard |
QL-CON-00-013 | construct-specific | 3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). | ring trial validation | EU reference method |
QL-CON-GH-tFreeze-F/GH-tFreeze-R | construct-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). | ring trial validation | national standard |
QL-CON-gat/T-pinII-F/gat/T-pinII-R | construct-specific | 61061, 73496, 98140, DP356043 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) | in-house validation | national standard |
QL-CON-cptiF2/tNOS-uni-R | construct-specific | Kefeng6, SGK321 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) | in-house validation | none |
QL-CON-00-008 | construct-specific | 2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). | ring trial validation | ISO standard |
QL-CON-CsVMV F/patsyn R | construct-specific | DAS44406, DAS68416, DAS81419 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QL-CON-35S-F/nptII-R | construct-specific | 23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). | in-house validation | none |
QL-CON-307-F/652-R | construct-specific | KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013). | in-house validation | none |
QL-CON-00-015 | construct-specific | Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC) | in-house validation | none |
QL-CON-00-011 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DHA, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3). | ring trial validation | ISO standard |
QL-ELE-T-g7-F/T-g7-R | element-specific | T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
QL-ELE-00-016 | element-specific | CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). | ring trial validation | ISO standard |
QL-ELE-00-026 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016). | ring trial validation | EU reference method |
QL-ELE-00-014 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) | ring trial validation | ISO standard |
QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |
QL-ELE-Cry1F-F2/Cry1Fr-n2 | element-specific | CS-cry1F_tr-BACTA |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) | in-house validation | none |
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 | element-specific | CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). | in-house validation | none |
QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
QL-ELE-BstarF-n2 /BstarR-n2 | element-specific | CS-barstar |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) | in-house validation | none |
QL-ELE-B'nase-F-n4/BNaseR-n3 | element-specific | CS-barnase |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). | in-house validation | none |
QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |
QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QL-ELE-P-FMV-F/P-FMV-R | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) | in-house validation | none |
QL-ELE-epsps 1-5'/epsps 3-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). | in-house validation | none |
QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
QL-TAX-OS-003 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) | ring trial validation | national standard |
QL-EVE-OS-001 | event-specific | LLRICE601 |
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). | in-house validation | none |
QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
QL-CON-00-007 | construct-specific | Huahui No.1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) | ring trial validation | ISO standard |
QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none |
QL-TAX-OS-002 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). | in-house validation | unknown |
QL-CON-00-012 | construct-specific | 55-1, 63-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) | ring trial validation | ISO standard |
QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |