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QL-EVE-375LibF/376LibR event-specific
Liberator
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6). ring trial validation national standard
QL-ELE-00-024 element-specific
T-rbcS_E9-PEA
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). ring trial validation EU reference method
QT-EVE-GM-017 event-specific
SYHT0H2
Quantitative real-time PCR (TaqMan) method for detection of soybean event SYHT0H2(EU-RL GMFF GMOMETHODS database as of September 19, 2016) ring trial validation EU reference method
QT-TAX-CP-001 taxon-specific
Carica papaya
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) ring trial validation EU reference method
QT-EVE-CP-001 event-specific
Huanong No. 1
Quantitative real-time (TaqMan) PCR method for detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 13, 2016). ring trial validation EU reference method
QL-EVE-CP-001 event-specific
Huanong No. 1
Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016) ring trial validation EU reference method
QT-EVE-GM-013 event-specific
DAS68416
Quantitative real-time PCR (TaqMan) method for the detection of soybean event DAS68416 (EU-RL GMFF GMOMETHODS database as of July 2, 2015) ring trial validation EU reference method
QL-TAX-ZM-002 taxon-specific
Zea mays
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-BN-003 taxon-specific
Brassica napus
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-GM-001 taxon-specific
Glycine max
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-020 element-specific
CS-cry1Ab-BACTU
Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-021 element-specific
CS-pat-STRVR
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-017 element-specific
P-Cauliflower mosaic virus
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-018 element-specific
T-nos-RHIRD
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-019 element-specific
CS-CP4epsps-RHIRD
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-ELE-00-022 element-specific
CS-bar-STRHY
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) ring trial validation unknown
QL-TAX-CP-001 taxon-specific
Carica papaya
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) ring trial validation EU reference method
QL-TAX-PS-001 taxon-specific
Pisum sativum
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) ring trial validation EU reference method
QL-ELE-00-023 element-specific
T-Cauliflower mosaic virus
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). ring trial validation EU reference method
QT-CON-00-003 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570). ring trial validation ISO standard
QT-EVE-GM-010 event-specific
MON87701
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-002 event-specific
MON87769
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87769 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GM-004 event-specific
A2704-12
Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014) ring trial validation EU reference method
QT-TAX-BN-001 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-BN-002 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4

ring trial validation EU reference method
QT-EVE-GH-009 event-specific
T304-40
Quantitative real-time PCR (TaqMan) method for detection of cotton event T304-40 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-TAX-ST-010 taxon-specific
Solanum tuberosum
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QL-EVE-ZM-001 event-specific
MON810
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). ring trial validation ISO standard
QT-EVE-GH-005 event-specific
MON15985
Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-019 event-specific
3272
Quantitative real-time PCR (TaqMan) method for detection of maize event 3272 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QL-CON-00-012 construct-specific
55-1, 63-1
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) ring trial validation ISO standard
QT-CON-00-004 construct-specific
DBN9936, MON80100, MON802, MON809, MON810
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002) ring trial validation ISO standard
QT-ELE-00-003 element-specific
CS-cry1Ab-BACTU
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) ring trial validation ISO standard
QT-EVE-GM-012 event-specific
MON87708
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87708 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-TAX-GM-001 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-EVE-ZM-004 event-specific
DAS40278
Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-010 event-specific
DAS1507
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1507 (EU-RL GMFF GMOMETHODS database as of March 17, 2014) ring trial validation EU reference method
QT-EVE-ZM-017 event-specific
LY038
Quantitative real-time PCR (TaqMan) method for detection of maize event LY038 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-016 event-specific
MON88017
Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-BN-009 event-specific
73496
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event 73496 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QL-TAX-SL-005 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). ring trial validation unknown
QL-BAC-27f/926R taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QL-BAC-16S-FW/16S-Rev taxon-specific
bacteria
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.

This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1).

ring trial validation national standard
QT-ELE-00-002 element-specific
CS-pat-STRVR
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) ring trial validation unknown
QT-TAX-ZM-001 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-SL-002 taxon-specific
Solanum lycopersicum
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) ring trial validation ISO standard
QT-TAX-OS-003 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) ring trial validation unknown
QL-EVE-BN-002 event-specific
GT73
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). ring trial validation ISO standard
QL-CON-00-001 construct-specific
GTS 40-3-2
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) ring trial validation ISO standard
QL-TAX-GM-002 taxon-specific
Glycine max
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). ring trial validation ISO standard
QL-CON-00-003 construct-specific
Bt10 maize, Bt11
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) ring trial validation ISO standard
QL-CON-00-002 construct-specific
Nema282F
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) ring trial validation ISO standard
QL-TAX-CMV-001 taxon-specific
Cauliflower mosaic virus
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). ring trial validation ISO standard
QL-TAX-AT-001 element-specific
CS-nos-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022). ring trial validation ISO standard
QL-CON-GH-tFreeze-F/GH-tFreeze-R construct-specific
AquAdvantage Salmon
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). ring trial validation national standard
QL-PLN-00-007 taxon-specific
all-plants
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) ring trial validation ISO standard
QT-EVE-BN-010 event-specific
MON88302
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-CON-00-006 construct-specific
Bt10 maize, Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) ring trial validation ISO standard
QL-TAX-SL-001 taxon-specific
Solanum lycopersicum
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). ring trial validation ISO standard
QT-EVE-ZM-003 event-specific
MON87427
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). ring trial validation EU reference method
QL-ELE-00-016 element-specific
CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). ring trial validation ISO standard
QT-EVE-GM-001 event-specific
FG72
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-013 event-specific
MIR604
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BV-001 event-specific
H7-1
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) ring trial validation EU reference method
QL-CON-00-010 construct-specific
FP967
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) ring trial validation ISO standard
QT-EVE-ZM-002 event-specific
5307
Quantitative real-time PCR (TaqMan) method for detection of maize event 5307 (EURL GMFF GMOMETHODS database as of 2014). ring trial validation EU reference method
QT-EVE-ZM-025 event-specific
MON87403
Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018) ring trial validation EU reference method
QT-EVE-ZM-028 event-specific
MZIR098
Quantitative real-time PCR (TaqMan) method for detection of MZIR098. (EURL GMFF GMOMETHODS database as of 2018). ring trial validation EU reference method
QT-TAX-ZM-005 taxon-specific
Zea mays
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). ring trial validation EU reference method
QT-EVE-ZM-026 event-specific
4114
Quantitative PCR method for detection of maize event 4114 (EURL GMFF GMOMETHODS database, as of May 24, 2018). ring trial validation EU reference method
QT-EVE-ZM-009 event-specific
MON863
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-024 event-specific
MON87411
Quantitative PCR method for detection of maize event MON87411 (EURL GMFF GMOMETHODS database, as of April 21, 2017) ring trial validation EU reference method
QT-TAX-ZM-004 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-EVE-ZM-001 event-specific
VCO1981
Quantitative real-time PCR (TaqMan) method for detection of maize event VCO-01981-5 (EURL GMFF GMOMETHODS database as of July 14, 2016) ring trial validation EU reference method
QT-EVE-GH-010 event-specific
MON88701
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) ring trial validation EU reference method
QT-EVE-GM-016 event-specific
MON87751
Quantitative PCR method for detection of soybean event MON87751 (EURL GMFF GMOMETHODS database as of April 21, 2017) ring trial validation EU reference method
QT-EVE-ZM-027 event-specific
MZHG0JG
Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018). ring trial validation EU reference method
QL-TAX-Acc1-F/Acc1-R taxon-specific
Medicago sativa
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018). ring trial validation national standard
QL-EVE-MS-KK179-F/KK179-R event-specific
KK179
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018). ring trial validation national standard
QL-EVE-MS-J163-F/J163-R event-specific
J163
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018). ring trial validation national standard
QL-EVE-MS-J101-F/J101-R event-specific
J101
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018) ring trial validation national standard
QT-EVE-ZM-029 event-specific
MON87419
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87419 (EU-RL GMFF GMOMETHODS database as of September 23, 2019) ring trial validation EU reference method
QT-EVE-GH-011 event-specific
DAS81910
Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019) ring trial validation EU reference method
QT-EVE-BN-011 event-specific
Ms11
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019). ring trial validation EU reference method
QL-EVE-EC-002 event-specific
PL73 E. coli (LM)
Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017) ring trial validation EU reference method
QL-EVE-EC-001 event-specific
PT73 E. coli (TM)
Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017) ring trial validation EU reference method
QT-EVE-OS-G6H1-F/G6H1-R event-specific
G6H1
Quantitative real-time PCR (TaqMan) method for detection of rice event G6H1 ring trial validation unknown
QT-EVE-BN-014 event-specific
MON94100
Quantitative real-time PCR (TaqMan) for detection of oilseed rape event MON94100 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) ring trial validation EU reference method
QL-ELE-00-026 element-specific
CS-bar-STRHY
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016). ring trial validation EU reference method
QT-TAX-ZM-008 taxon-specific
Zea mays
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-EVE-GM-003 event-specific
MON87705
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87705 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-TAX-GM-002 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-EVE-ZM-005 event-specific
MON87460
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-ZM-006 event-specific
Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570) ring trial validation ISO standard
QT-EVE-ZM-007 event-specific
GA21
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-011 event-specific
T25
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-TAX-ZM-003 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-012 event-specific
DAS59122
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-014 event-specific
GA21
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-015 event-specific
Bt11
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
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