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QT-EVE-ZM-005 event-specific
MON87460
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-ZM-016 event-specific
MON88017
Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-018 event-specific
MON89034
Quantitative real-time PCR (TaqMan) method for detection of maize event MON89034 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-ZM-036 event-specific
MON95275
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95275 (EU-RL GMFF GMOMETHODS database as of February 15, 2024) ring trial validation EU reference method
QT-EVE-ZM-033 event-specific
MON95379
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95379 (EU-RL GMFF GMOMETHODS database as of December 20, 2022) ring trial validation EU reference method
QT-EVE-ZM-008 event-specific
NK603
Quantitative real-time PCR (TaqMan) method for detection of maize event NK603 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-ZM-011 event-specific
T25
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-CON-00-005 construct-specific
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) ring trial validation ISO standard
QT-EVE-ZM-001 event-specific
VCO1981
Quantitative real-time PCR (TaqMan) method for detection of maize event VCO-01981-5 (EURL GMFF GMOMETHODS database as of July 14, 2016) ring trial validation EU reference method
QT-TAX-ZM-006 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) ring trial validation ISO standard
QT-TAX-zSSIIb-3F/zSSIIb-4R taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) unknown unknown
QT-TAX-BN-001 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-BN-012 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-TAX-BN-003 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) ring trial validation EU reference method
QT-TAX-BN-002 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4

ring trial validation EU reference method
QT-EVE-BN-009 event-specific
73496
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event 73496 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-004 event-specific
GT73
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-012 event-specific
LBFLFK
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022) ring trial validation EU reference method
QT-EVE-BN-013 event-specific
LBFLFK
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) ring trial validation EU reference method
QT-EVE-BN-010 event-specific
MON88302
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-EVE-BN-005 event-specific
Ms1
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) in-house validation EU reference method
QT-EVE-BN-011 event-specific
Ms11
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019). ring trial validation EU reference method
QT-EVE-BN-002 event-specific
Ms8
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-006 event-specific
Rf1
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) in-house validation EU reference method
QT-EVE-BN-007 event-specific
Rf2
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) in-house validation unknown
QT-EVE-BN-003 event-specific
Rf3
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-001 event-specific
T45
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-BN-008 event-specific
Topas 19/2
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) in-house validation EU reference method
QT-ELE-00-002 element-specific
CS-pat-STRVR
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) ring trial validation unknown
QT-TAX-ST-010 taxon-specific
Solanum tuberosum
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QT-EVE-ST-001 event-specific
EH92-527-1
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) ring trial validation EU reference method
QT-TAX-GH-021 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method
QT-EVE-OS-G6H1-F/G6H1-R event-specific
G6H1
Quantitative real-time PCR (TaqMan) method for detection of rice event G6H1 ring trial validation unknown
QT-EVE-OS-002 event-specific
LLRICE62
Quantitative real-time PCR (TaqMan) method for detection of rice event LLRICE62 (EU-RL GMFF GMOMETHODS database as of January 01, 2014) ring trial validation EU reference method
QT-TAX-OS-002 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) ring trial validation EU reference method
QT-TAX-OS-003 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) ring trial validation unknown
QT-EVE-GM-004 event-specific
A2704-12
Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014) ring trial validation EU reference method
QT-EVE-GM-007 event-specific
A5547-127
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-011 event-specific
CV127
Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-019 event-specific
DBN9004
Quantitative real-time PCR (TaqMan) method for detection of soybean event DBN9004 (EU-RL GMFF GMOMETHODS database as of November 8, 2023). ring trial validation EU reference method
QT-EVE-GM-008 event-specific
DP305423
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP305423 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-009 event-specific
DP356043
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-001 event-specific
FG72
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GM-018 event-specific
GMB151
Quantitative real-time PCR (TaqMan) method for detection of soybean event GMB151 (EU-RL GMFF GMOMETHODS database as of August 10, 2020) ring trial validation EU reference method
QT-EVE-GM-005 event-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-CON-00-002 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (Hird et al., 2003). ring trial validation national standard
QT-CON-00-001 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO 21570) ring trial validation ISO standard
QT-CON-00-003 construct-specific
GTS 40-3-2
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570). ring trial validation ISO standard
QT-EVE-GM-010 event-specific
MON87701
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-003 event-specific
MON87705
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87705 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GM-012 event-specific
MON87708
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87708 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) ring trial validation EU reference method
QT-EVE-GM-002 event-specific
MON87769
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87769 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) ring trial validation EU reference method
QT-EVE-GM-006 event-specific
MON89788
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON89788 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) ring trial validation EU reference method
QT-EVE-GM-017 event-specific
SYHT0H2
Quantitative real-time PCR (TaqMan) method for detection of soybean event SYHT0H2(EU-RL GMFF GMOMETHODS database as of September 19, 2016) ring trial validation EU reference method
QT-TAX-GM-009 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) ring trial validation EU reference method
QT-TAX-GM-020 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-GM-003 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-TAX-GM-001 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-TAX-GM-007 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene ring trial validation ISO standard
QT-TAX-GM-004 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003). ring trial validation unknown
QT-TAX-GM-002 taxon-specific
Glycine max
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-EVE-BV-001 event-specific
H7-1
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) ring trial validation EU reference method
QT-TAX-BV-013 taxon-specific
Beta vulgaris
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) ring trial validation EU reference method
QT-ELE-00-003 element-specific
CS-cry1Ab-BACTU
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) ring trial validation ISO standard
QT-ELE-P35S 1-5'/P35S 1-3' element-specific
P-Cauliflower mosaic virus
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) ring trial validation national standard
QT-TAX-wx012-5'/wx012-3' taxon-specific
Triticum aestivum
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) in-house validation none
QT-TAX-ZM-011 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) ring trial validation EU reference method
QT-TAX-ZM-002 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard
QT-TAX-SPS primer f/SPS primer r taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) ring trial validation ISO standard
QT-TAX-SL-002 taxon-specific
Solanum lycopersicum
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) ring trial validation ISO standard
QT-EVE-ZM-004 event-specific
DAS40278
Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method
QT-EVE-GH-005 event-specific
MON15985
Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-TAX-FatA primer1/FatA primer2 taxon-specific
Brassica napus
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

in-house validation none
QT-TAX-OS-017 taxon-specific
Oryza sativa
Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014) ring trial validation EU reference method
QT-EVE-GM-013 event-specific
DAS68416
Quantitative real-time PCR (TaqMan) method for the detection of soybean event DAS68416 (EU-RL GMFF GMOMETHODS database as of July 2, 2015) ring trial validation EU reference method
QT-TAX-GH-019 taxon-specific
Gossypium hirsutum
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
QT-EVE-ZM-034 event-specific
DP202216
Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023) ring trial validation EU reference method
QT-TAX-ZM-008 taxon-specific
Zea mays
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method
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