Detection methods search result
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Name | Type | Target | Description | Validation | Standardisation |
---|---|---|---|---|---|
QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
QT-TAX-zSSIIb-3F/zSSIIb-4R | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) | unknown | unknown |
QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |
QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). | unknown | none |
QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | A1-DFR Petunias, RL01-15, RL01-17 |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). | unknown | none |
QL-EVE-ZM-P3waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
QL-EVE-ZM-P1waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |
QL-CON-35S-F/nptII-R | construct-specific | 23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). | in-house validation | none |
QL-CON-307-F/652-R | construct-specific | KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013). | in-house validation | none |
QL-TAX-LU-001 | taxon-specific | Linum usitatissimum |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) | in-house validation | none |
QL-CON-cptiF2/tNOS-uni-R | construct-specific | Kefeng6, SGK321 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) | in-house validation | none |
QL-EVE-MDB110/SHA016 | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) | in-house validation | EU reference method |
QL-ELE-T-g7-F/T-g7-R | element-specific | T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
QL-ELE-T-OCS-F/T-OCS-R | element-specific | T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
QL-ELE-P-TA29-F/P-TA29-R | element-specific | P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) | in-house validation | none |
QL-ELE-P-SSuAra-F/P-SSuAra-R | element-specific | P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) | in-house validation | none |
QL-ELE-P-FMV-F/P-FMV-R | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) | in-house validation | none |
QL-ELE-P-Ubi-F/P-Ubi-R | element-specific | P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) | in-house validation | none |
QL-EVE-SQ0718/SQ0719 | event-specific | MON71800 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013). | in-house validation | none |
QL-CON-gat/T-pinII-F/gat/T-pinII-R | construct-specific | 61061, 73496, 98140, DP356043 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) | in-house validation | national standard |
QL-EVE-ZM-002 | event-specific | Bt10 maize |
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) | in-house validation | none |
QT-EVE-BN-007 | event-specific | Rf2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) | in-house validation | unknown |
QL-ELE-P-nos-F/P-nos-R | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
QL-EVE-Oxy-3F/Oxy-4R | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) | in-house validation | none |
QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
QL-EVE-DC-003 | event-specific | 25958 |
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
QL-EVE-DC-004 | event-specific | 26407 |
Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
QL-EVE-DC-005 | event-specific | 27531 |
Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016) | in-house validation | EU reference method |
QL-EVE-DC-001 | event-specific | 123.2.38 |
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). | in-house validation | EU reference method |
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 | element-specific | CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). | in-house validation | none |
QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
QT-EVE-ZM-023 | event-specific | Bt176 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014). | in-house validation | EU reference method |
QL-EVE-TA-MON71200 | event-specific | MON71200 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). | in-house validation | EU reference method |
QL-EVE-DC-006 | event-specific | 123.8.8 |
Qualitative conventional PCR method for detection of carnation event 123.8.8 (EURL GMFF GMOMETHODS database as of December 15, 2017). | in-house validation | EU reference method |
QT-EVE-HF-1F/HF-2R | event-specific | Huafan No 1 |
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) | in-house validation | unknown |
QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
QL-CON-00-015 | construct-specific | Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC) | in-house validation | none |
QT-TAX-FatA primer1/FatA primer2 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
in-house validation | none |
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
QT-EVE-BN-005 | event-specific | Ms1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
QT-EVE-BN-006 | event-specific | Rf1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
QT-EVE-BN-008 | event-specific | Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | in-house validation | EU reference method |
QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 | element-specific | CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). | in-house validation | none |
QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
QT-TAX-wx012-5'/wx012-3' | taxon-specific | Triticum aestivum |
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) | in-house validation | none |
QL-EVE-OS-001 | event-specific | LLRICE601 |
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). | in-house validation | none |
QL-TAX-OS-002 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). | in-house validation | unknown |
QL-ELE-PFMV 1-5'/PFMV 1-3' | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) | in-house validation | none |
QL-ELE-epsps 1-5'/epsps 3-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). | in-house validation | none |
QL-ELE-Cry1F-F2/Cry1Fr-n2 | element-specific | CS-cry1F_tr-BACTA |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) | in-house validation | none |
QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none |
QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none |
QL-ELE-B'nase-F-n4/BNaseR-n3 | element-specific | CS-barnase |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). | in-house validation | none |
QL-ELE-BstarF-n2 /BstarR-n2 | element-specific | CS-barstar |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) | in-house validation | none |
QL-TAX-CaMVF/CaMVR | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) | in-house validation | none |
QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |
QL-ELE-npt 1-5'/npt 1-3' | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). | in-house validation | none |
QT-EVE-ZM-034 | event-specific | DP202216 |
Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023) | ring trial validation | EU reference method |
QT-EVE-GH-012 | event-specific | COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) | ring trial validation | EU reference method |
QT-EVE-BN-012 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
QT-EVE-BN-013 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
QT-EVE-ZM-031 | event-specific | DP915635 |
Quantitative PCR method for detection of maize event DP915635 (EU-RL GMFF GMOMETHODS database as of May 5, 2023). | ring trial validation | EU reference method |
QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-GM-003 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-EVE-GM-018 | event-specific | GMB151 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GMB151 (EU-RL GMFF GMOMETHODS database as of August 10, 2020) | ring trial validation | EU reference method |
QT-EVE-GH-013 | event-specific | GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB811 (EU-RL GMFF GMOMETHODS database as of August 5, 2020) | ring trial validation | EU reference method |
QL-CON-CsVMV F/patsyn R | construct-specific | DAS44406, DAS68416, DAS81419 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QT-TAX-BN-003 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) | ring trial validation | EU reference method |
QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
QT-TAX-GM-005 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
QT-EVE-GM-019 | event-specific | DBN9004 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DBN9004 (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
QT-EVE-DBN9501-qF/DBN9501-qR | event-specific | DBN9501 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DBN9501 (Wang et al., 2023) | ring trial validation | unknown |
QT-EVE-ZM-035 | event-specific | DP23211 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DP23211 (EU-RL GMFF GMOMETHODS database as of February 15, 2024) | ring trial validation | EU reference method |
QT-EVE-ZM-036 | event-specific | MON95275 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95275 (EU-RL GMFF GMOMETHODS database as of February 15, 2024) | ring trial validation | EU reference method |
QT-EVE-ZM-030 | event-specific | MON87429 |
Quantitative PCR method for detection of maize event MON87429 (EU-RL GMFF GMOMETHODS database as of August 30, 2021) | ring trial validation | EU reference method |
QT-EVE-ZM-033 | event-specific | MON95379 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95379 (EU-RL GMFF GMOMETHODS database as of December 20, 2022) | ring trial validation | EU reference method |
QT-EVE-GM-015 | event-specific | DAS44406 |
Quantitative PCR method for detection of soybean event DAS44406 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
QT-TAX-OS-002 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
QT-EVE-OS-001 | event-specific | Golden Rice 2 |
Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
QL-CON-CP1/pFMV2 | construct-specific | GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). | ring trial validation | national standard |
QL-ELE-00-025 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. | ring trial validation | EU reference method |
QL-ELE-00-015 | element-specific | E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). | ring trial validation | ISO standard |
QL-ELE-00-008 | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) | ring trial validation | ISO standard |
QT-EVE-GM-014 | event-specific | DAS81419 |
Quantitative PCR method for detection of soybean event DAS81419 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
QL-CON-PGS-bar-A2/PGS-bar-B2 | construct-specific | Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea) |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). | ring trial validation | national standard |
QL-ELE-00-024 | element-specific | T-rbcS_E9-PEA |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). | ring trial validation | EU reference method |