Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | A1-DFR Petunias, RL01-15, RL01-17 |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QT-TAX-zSSIIb-3F/zSSIIb-4R | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) | unknown | unknown |
| QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). | unknown | none |
| QL-EVE-ZM-P3waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QL-EVE-ZM-P1waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
| QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |
| QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). | unknown | none |
| QL-EVE-Oxy-3F/Oxy-4R | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) | in-house validation | none |
| QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
| QL-EVE-DC-003 | event-specific | 25958 |
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-004 | event-specific | 26407 |
Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-005 | event-specific | 27531 |
Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-001 | event-specific | 123.2.38 |
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). | in-house validation | EU reference method |
| QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
| QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
| QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
| QT-EVE-ZM-023 | event-specific | Bt176 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014). | in-house validation | EU reference method |
| QL-EVE-TA-MON71200 | event-specific | MON71200 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). | in-house validation | EU reference method |
| QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 | element-specific | CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). | in-house validation | none |
| QL-EVE-DC-006 | event-specific | 123.8.8 |
Qualitative conventional PCR method for detection of carnation event 123.8.8 (EURL GMFF GMOMETHODS database as of December 15, 2017). | in-house validation | EU reference method |
| QT-EVE-HF-1F/HF-2R | event-specific | Huafan No 1 |
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) | in-house validation | unknown |
| QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
| QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
| QL-CON-00-015 | construct-specific | Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC) | in-house validation | none |
| QL-CON-35S-F/nptII-R | construct-specific | 23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). | in-house validation | none |
| QL-CON-307-F/652-R | construct-specific | KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013). | in-house validation | none |
| QL-TAX-LU-001 | taxon-specific | Linum usitatissimum |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) | in-house validation | none |
| QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |
| QL-CON-cptiF2/tNOS-uni-R | construct-specific | Kefeng6, SGK321 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) | in-house validation | none |
| QL-EVE-MDB110/SHA016 | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) | in-house validation | EU reference method |
| QL-ELE-T-g7-F/T-g7-R | element-specific | T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-OCS-F/T-OCS-R | element-specific | T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
| QL-ELE-P-TA29-F/P-TA29-R | element-specific | P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-SSuAra-F/P-SSuAra-R | element-specific | P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-FMV-F/P-FMV-R | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-Ubi-F/P-Ubi-R | element-specific | P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) | in-house validation | none |
| QL-EVE-SQ0718/SQ0719 | event-specific | MON71800 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013). | in-house validation | none |
| QL-CON-gat/T-pinII-F/gat/T-pinII-R | construct-specific | 61061, 73496, 98140, DP356043 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) | in-house validation | national standard |
| QT-EVE-BN-007 | event-specific | Rf2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) | in-house validation | unknown |
| QL-ELE-P-nos-F/P-nos-R | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
| QL-EVE-ZM-002 | event-specific | Bt10 maize |
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) | in-house validation | none |
| QT-TAX-FatA primer1/FatA primer2 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
in-house validation | none |
| QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 | element-specific | CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). | in-house validation | none |
| QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
| QT-TAX-wx012-5'/wx012-3' | taxon-specific | Triticum aestivum |
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) | in-house validation | none |
| QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
| QT-EVE-BN-005 | event-specific | Ms1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-006 | event-specific | Rf1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-008 | event-specific | Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | in-house validation | EU reference method |
| QL-EVE-OS-001 | event-specific | LLRICE601 |
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). | in-house validation | none |
| QL-TAX-OS-002 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). | in-house validation | unknown |
| QL-ELE-PFMV 1-5'/PFMV 1-3' | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) | in-house validation | none |
| QL-ELE-epsps 1-5'/epsps 3-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-Cry1F-F2/Cry1Fr-n2 | element-specific | CS-cry1F_tr-BACTA |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) | in-house validation | none |
| QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none |
| QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-npt 1-5'/npt 1-3' | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). | in-house validation | none |
| QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none |
| QL-ELE-B'nase-F-n4/BNaseR-n3 | element-specific | CS-barnase |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-BstarF-n2 /BstarR-n2 | element-specific | CS-barstar |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) | in-house validation | none |
| QL-TAX-CaMVF/CaMVR | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) | in-house validation | none |
| QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QT-ELE-00-002 | element-specific | CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) | ring trial validation | unknown |
| QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QT-TAX-SL-002 | taxon-specific | Solanum lycopersicum |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) | ring trial validation | ISO standard |
| QT-TAX-OS-003 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) | ring trial validation | unknown |
| QL-EVE-BN-002 | event-specific | GT73 |
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). | ring trial validation | ISO standard |
| QL-CON-00-001 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-CON-00-003 | construct-specific | Bt10 maize, Bt11 |
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-002 | construct-specific | Nema282F |
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
| QT-EVE-ZM-003 | event-specific | MON87427 |
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). | ring trial validation | EU reference method |
| QL-TAX-CMV-001 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-TAX-AT-001 | element-specific | CS-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-CON-GH-tFreeze-F/GH-tFreeze-R | construct-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). | ring trial validation | national standard |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QT-EVE-BN-010 | event-specific | MON88302 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
| QT-CON-00-006 | construct-specific | Bt10 maize, Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-GM-003 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-EVE-GM-018 | event-specific | GMB151 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GMB151 (EU-RL GMFF GMOMETHODS database as of August 10, 2020) | ring trial validation | EU reference method |
| QT-EVE-GH-013 | event-specific | GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB811 (EU-RL GMFF GMOMETHODS database as of August 5, 2020) | ring trial validation | EU reference method |
| QT-EVE-ZM-009 | event-specific | MON863 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-034 | event-specific | DP202216 |
Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023) | ring trial validation | EU reference method |
| QT-EVE-GH-012 | event-specific | COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) | ring trial validation | EU reference method |
| QT-EVE-BN-012 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
| QT-EVE-BN-013 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
| QT-EVE-ZM-031 | event-specific | DP915635 |
Quantitative PCR method for detection of maize event DP915635 (EU-RL GMFF GMOMETHODS database as of May 5, 2023). | ring trial validation | EU reference method |
| QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |