Detection methods search result
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Name | Type | Target | Description | Validation | Standardisation |
---|---|---|---|---|---|
QT-EVE-ZM-007 | event-specific | GA21 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-011 | event-specific | T25 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-012 | event-specific | DAS59122 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-014 | event-specific | GA21 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-015 | event-specific | Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-018 | event-specific | MON89034 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON89034 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-021 | event-specific | 98140 |
Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-022 | event-specific | MIR162 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014) | ring trial validation | EU reference method |
QT-EVE-GM-005 | event-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-GM-006 | event-specific | MON89788 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON89788 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
QT-EVE-GM-007 | event-specific | A5547-127 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-GM-009 | event-specific | DP356043 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-001a | event-specific | 281-24-236 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-002 | event-specific | LLCotton25 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-GH-003 | event-specific | MON1445 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-EVE-ZM-020 | event-specific | MON810 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570) | ring trial validation | ISO standard |
QT-TAX-GM-005 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
QT-TAX-zSSIIb-3F/zSSIIb-4R | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) | unknown | unknown |
QT-TAX-BN-003 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) | ring trial validation | EU reference method |
QL-TAX-LU-001 | taxon-specific | Linum usitatissimum |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) | in-house validation | none |
QT-TAX-OS-002 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
QT-TAX-CP-001 | taxon-specific | Carica papaya |
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) | ring trial validation | EU reference method |
QL-TAX-ZM-002 | taxon-specific | Zea mays |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
QL-TAX-BN-003 | taxon-specific | Brassica napus |
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
QL-TAX-GM-001 | taxon-specific | Glycine max |
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
QL-TAX-CP-001 | taxon-specific | Carica papaya |
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) | ring trial validation | EU reference method |
QL-TAX-PS-001 | taxon-specific | Pisum sativum |
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) | ring trial validation | EU reference method |
QT-TAX-BN-001 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-BN-002 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4 |
ring trial validation | EU reference method |
QT-TAX-ST-010 | taxon-specific | Solanum tuberosum |
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) | ring trial validation | EU reference method |
QL-TAX-SL-005 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). | ring trial validation | unknown |
QT-TAX-GM-001 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene | ring trial validation | ISO standard |
QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
QT-TAX-SL-002 | taxon-specific | Solanum lycopersicum |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) | ring trial validation | ISO standard |
QT-TAX-OS-003 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) | ring trial validation | unknown |
QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
QL-TAX-CMV-001 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
QT-TAX-ZM-004 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QL-TAX-Acc1-F/Acc1-R | taxon-specific | Medicago sativa |
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018). | ring trial validation | national standard |
QT-TAX-ZM-005 | taxon-specific | Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). | ring trial validation | EU reference method |
QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-GM-003 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-ZM-008 | taxon-specific | Zea mays |
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-GH-015 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-TAX-GH-019 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-BN-012 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-TAX-FatA primer1/FatA primer2 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
in-house validation | none |
QT-TAX-GM-007 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene | ring trial validation | ISO standard |
QT-TAX-ZM-006 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) | ring trial validation | ISO standard |
QL-TAX-OS-006 | taxon-specific | Oryza sativa |
Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009). | ring trial validation | unknown |
QT-TAX-GM-004 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003). | ring trial validation | unknown |
QT-TAX-BV-013 | taxon-specific | Beta vulgaris |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014) | ring trial validation | EU reference method |
QT-TAX-GM-009 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) | ring trial validation | EU reference method |
QL-TAX-ZM-003 | taxon-specific | Zea mays |
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) | ring trial validation | ISO standard |
QL-TAX-OS-003 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) | ring trial validation | national standard |
QT-TAX-wx012-5'/wx012-3' | taxon-specific | Triticum aestivum |
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) | in-house validation | none |
QL-TAX-BN-004 | taxon-specific | Brassica napus |
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). | ring trial validation | unknown |
QT-TAX-OS-017 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
QT-TAX-ZM-002 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
QT-TAX-ZM-011 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) | ring trial validation | EU reference method |
QT-TAX-ZM-014 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QL-TAX-OS-002 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). | in-house validation | unknown |
QL-TAX-GM-008 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) | ring trial validation | ISO standard |
QT-TAX-SPS primer f/SPS primer r | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) | ring trial validation | ISO standard |
QL-TAX-CaMVF/CaMVR | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) | in-house validation | none |
QT-TAX-GM-002 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
QT-TAX-ZM-003 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
QT-TAX-GM-020 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
QT-TAX-GH-016 | taxon-specific | Gossypium hirsutum |
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-TAX-GH-021 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
QT-TAX-GH-018 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |