Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QT-CON-00-004 | construct-specific | DBN9936, MON80100, MON802, MON809, MON810 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-CON-00-001 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-003 | construct-specific | Bt10 maize, Bt11 |
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-002 | construct-specific | Nema282F |
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | A1-DFR Petunias, RL01-15, RL01-17 |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
| QL-CON-GH-tFreeze-F/GH-tFreeze-R | construct-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). | ring trial validation | national standard |
| QT-CON-00-006 | construct-specific | Bt10 maize, Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-CON-00-010 | construct-specific | FP967 |
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) | ring trial validation | ISO standard |
| QL-CON-00-015 | construct-specific | Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC) | in-house validation | none |
| QL-CON-CsVMV F/patsyn R | construct-specific | DAS44406, DAS68416, DAS81419 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-CON-35S-F/nptII-R | construct-specific | 23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010). | in-house validation | none |
| QL-CON-307-F/652-R | construct-specific | KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013). | in-house validation | none |
| QL-CON-CP1/pFMV2 | construct-specific | GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). | ring trial validation | national standard |
| QL-CON-PGS-bar-A2/PGS-bar-B2 | construct-specific | Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea) |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). | ring trial validation | national standard |
| QL-CON-cptiF2/tNOS-uni-R | construct-specific | Kefeng6, SGK321 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) | in-house validation | none |
| QL-CON-gat/T-pinII-F/gat/T-pinII-R | construct-specific | 61061, 73496, 98140, DP356043 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016) | in-house validation | national standard |
| QT-CON-00-003 | construct-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570). | ring trial validation | ISO standard |
| QL-CON-00-012 | construct-specific | 55-1, 63-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-013 | construct-specific | 3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). | ring trial validation | EU reference method |
| QL-CON-00-014 | construct-specific | 1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) | ring trial validation | ISO standard |
| QT-CON-00-001 | construct-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO 21570) | ring trial validation | ISO standard |
| QT-CON-00-002 | construct-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (Hird et al., 2003). | ring trial validation | national standard |
| QT-CON-00-007 | construct-specific | B827, Bt176 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QT-CON-00-005 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-CON-00-011 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DHA, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3). | ring trial validation | ISO standard |
| QT-CON-00-008 | construct-specific | DAS44406, FG72, GA21, GHB614, GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002) | ring trial validation | ISO standard |
| QL-CON-00-004 | construct-specific | Bt176 |
Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-005 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-006 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-007 | construct-specific | Huahui No.1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) | ring trial validation | ISO standard |
| QL-CON-00-008 | construct-specific | 2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). | ring trial validation | ISO standard |
| QT-ELE-00-003 | element-specific | CS-cry1Ab-BACTU |
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) | ring trial validation | ISO standard |
| QL-ELE-P-nos-F/P-nos-R | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
| QT-ELE-00-002 | element-specific | CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) | ring trial validation | unknown |
| QL-TAX-AT-001 | element-specific | CS-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 | element-specific | CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). | in-house validation | none |
| QL-ELE-00-016 | element-specific | CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). | ring trial validation | ISO standard |
| QL-ELE-00-026 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016). | ring trial validation | EU reference method |
| QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QL-ELE-00-025 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. | ring trial validation | EU reference method |
| QL-ELE-00-015 | element-specific | E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). | ring trial validation | ISO standard |
| QL-ELE-00-008 | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) | ring trial validation | ISO standard |
| QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |
| QL-ELE-T-g7-F/T-g7-R | element-specific | T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-OCS-F/T-OCS-R | element-specific | T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-00-024 | element-specific | T-rbcS_E9-PEA |
Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017). | ring trial validation | EU reference method |
| QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
| QL-ELE-P-TA29-F/P-TA29-R | element-specific | P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-SSuAra-F/P-SSuAra-R | element-specific | P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-FMV-F/P-FMV-R | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-Ubi-F/P-Ubi-R | element-specific | P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) | in-house validation | none |
| QL-ELE-00-020 | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-021 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-017 | element-specific | P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-018 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-019 | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-022 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). | unknown | none |
| QL-ELE-00-023 | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). | ring trial validation | EU reference method |
| QL-ELE-00-012 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
| QT-ELE-P35S 1-5'/P35S 1-3' | element-specific | P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) | ring trial validation | national standard |
| QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 | element-specific | CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). | in-house validation | none |
| QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
| QT-ELE-00-001 | element-specific | P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) | ring trial validation | unknown |
| QT-ELE-00-004 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) | ring trial validation | ISO standard |
| QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
| QL-ELE-00-001 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) | ring trial validation | national standard |
| QL-ELE-00-002 | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-00-003 | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) | ring trial validation | national standard |
| QL-ELE-00-004 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) | ring trial validation | ISO standard |
| QL-ELE-00-005 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-ELE-00-006 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) | ring trial validation | national standard |
| QL-ELE-00-007 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) | ring trial validation | national standard |
| QL-ELE-00-009 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) | ring trial validation | ISO standard |
| QL-ELE-00-010 | element-specific | E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) | ring trial validation | ISO standard |
| QL-ELE-00-011 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). | ring trial validation | ISO standard |
| QL-ELE-00-013 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-00-014 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) | ring trial validation | ISO standard |
| QT-ELE-NOS ter 2-5' /NOS ter 2-3' | element-specific | T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) | ring trial validation | none |
| QL-ELE-PFMV 1-5'/PFMV 1-3' | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) | in-house validation | none |
| QL-ELE-epsps 1-5'/epsps 3-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-Cry1F-F2/Cry1Fr-n2 | element-specific | CS-cry1F_tr-BACTA |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) | in-house validation | none |
| QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none |
| QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-npt 1-5'/npt 1-3' | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). | in-house validation | none |
| QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none |
| QL-ELE-B'nase-F-n4/BNaseR-n3 | element-specific | CS-barnase |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-BstarF-n2 /BstarR-n2 | element-specific | CS-barstar |
Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006) | in-house validation | none |
| QT-EVE-GM-012 | event-specific | MON87708 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87708 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
| QL-EVE-ZM-002 | event-specific | Bt10 maize |
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) | in-house validation | none |
| QT-EVE-BN-007 | event-specific | Rf2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) | in-house validation | unknown |
| QT-EVE-ZM-004 | event-specific | DAS40278 |
Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-019 | event-specific | 3272 |
Quantitative real-time PCR (TaqMan) method for detection of maize event 3272 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-010 | event-specific | DAS1507 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1507 (EU-RL GMFF GMOMETHODS database as of March 17, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-017 | event-specific | LY038 |
Quantitative real-time PCR (TaqMan) method for detection of maize event LY038 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-016 | event-specific | MON88017 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-009 | event-specific | 73496 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event 73496 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-019 | event-specific | DBN9004 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DBN9004 (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
| QT-EVE-DBN9501-qF/DBN9501-qR | event-specific | DBN9501 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DBN9501 (Wang et al., 2023) | ring trial validation | unknown |
| QT-EVE-ZM-035 | event-specific | DP23211 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DP23211 (EU-RL GMFF GMOMETHODS database as of February 15, 2024) | ring trial validation | EU reference method |
| QT-EVE-ZM-036 | event-specific | MON95275 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95275 (EU-RL GMFF GMOMETHODS database as of February 15, 2024) | ring trial validation | EU reference method |
| QL-EVE-Oxy-3F/Oxy-4R | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) | in-house validation | none |
| QL-EVE-BN-002 | event-specific | GT73 |
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). | ring trial validation | ISO standard |
| QT-EVE-ZM-003 | event-specific | MON87427 |
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). | ring trial validation | EU reference method |
| QL-EVE-DC-003 | event-specific | 25958 |
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-004 | event-specific | 26407 |
Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-005 | event-specific | 27531 |
Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-001 | event-specific | 123.2.38 |
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). | in-house validation | EU reference method |
| QT-EVE-BN-010 | event-specific | MON88302 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event MON88302 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-009 | event-specific | MON863 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-023 | event-specific | Bt176 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014). | in-house validation | EU reference method |
| QT-EVE-GM-001 | event-specific | FG72 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-013 | event-specific | MIR604 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BV-001 | event-specific | H7-1 |
Quantitative real-time PCR (TaqMan) method for detection of sugar beet event H7-1 (EU-RL GMFF GMOMETHODS database as March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-002 | event-specific | 5307 |
Quantitative real-time PCR (TaqMan) method for detection of maize event 5307 (EURL GMFF GMOMETHODS database as of 2014). | ring trial validation | EU reference method |
| QT-EVE-ZM-025 | event-specific | MON87403 |
Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018) | ring trial validation | EU reference method |
| QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). | unknown | none |
| QL-EVE-TA-MON71200 | event-specific | MON71200 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). | in-house validation | EU reference method |
| QT-EVE-ZM-028 | event-specific | MZIR098 |
Quantitative real-time PCR (TaqMan) method for detection of MZIR098. (EURL GMFF GMOMETHODS database as of 2018). | ring trial validation | EU reference method |
| QT-EVE-ZM-026 | event-specific | 4114 |
Quantitative PCR method for detection of maize event 4114 (EURL GMFF GMOMETHODS database, as of May 24, 2018). | ring trial validation | EU reference method |
| QT-EVE-ZM-024 | event-specific | MON87411 |
Quantitative PCR method for detection of maize event MON87411 (EURL GMFF GMOMETHODS database, as of April 21, 2017) | ring trial validation | EU reference method |
| QT-EVE-ZM-001 | event-specific | VCO1981 |
Quantitative real-time PCR (TaqMan) method for detection of maize event VCO-01981-5 (EURL GMFF GMOMETHODS database as of July 14, 2016) | ring trial validation | EU reference method |
| QT-EVE-GH-010 | event-specific | MON88701 |
Quantitative PCR method for detection of cotton event MON88701 (EURL GMFF GMOMETHODS database as of April 21, 2017) | ring trial validation | EU reference method |
| QT-EVE-GM-016 | event-specific | MON87751 |
Quantitative PCR method for detection of soybean event MON87751 (EURL GMFF GMOMETHODS database as of April 21, 2017) | ring trial validation | EU reference method |
| QT-EVE-ZM-027 | event-specific | MZHG0JG |
Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018). | ring trial validation | EU reference method |
| QL-EVE-MS-KK179-F/KK179-R | event-specific | KK179 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-J163-F/J163-R | event-specific | J163 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-J101-F/J101-R | event-specific | J101 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018) | ring trial validation | national standard |
| QT-EVE-ZM-029 | event-specific | MON87419 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87419 (EU-RL GMFF GMOMETHODS database as of September 23, 2019) | ring trial validation | EU reference method |
| QT-EVE-GH-011 | event-specific | DAS81910 |
Quantitative PCR method for detection of cotton event DAS81910 (EURL GMFF GMOMETHODS database as of January 10, 2019) | ring trial validation | EU reference method |
| QT-EVE-BN-011 | event-specific | Ms11 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019). | ring trial validation | EU reference method |
| QL-EVE-EC-002 | event-specific | PL73 E. coli (LM) |
Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017) | ring trial validation | EU reference method |
| QL-EVE-EC-001 | event-specific | PT73 E. coli (TM) |
Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017) | ring trial validation | EU reference method |
| QL-EVE-DC-006 | event-specific | 123.8.8 |
Qualitative conventional PCR method for detection of carnation event 123.8.8 (EURL GMFF GMOMETHODS database as of December 15, 2017). | in-house validation | EU reference method |
| QT-EVE-HF-1F/HF-2R | event-specific | Huafan No 1 |
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) | in-house validation | unknown |
| QT-EVE-OS-G6H1-F/G6H1-R | event-specific | G6H1 |
Quantitative real-time PCR (TaqMan) method for detection of rice event G6H1 | ring trial validation | unknown |
| QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
| QL-EVE-ZM-P3waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QL-EVE-ZM-P1waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QT-EVE-BN-014 | event-specific | MON94100 |
Quantitative real-time PCR (TaqMan) for detection of oilseed rape event MON94100 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
| QT-EVE-ZM-034 | event-specific | DP202216 |
Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023) | ring trial validation | EU reference method |
| QT-EVE-GH-012 | event-specific | COT102 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020) | ring trial validation | EU reference method |
| QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
| QT-EVE-BN-012 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
| QT-EVE-BN-013 | event-specific | LBFLFK |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) | ring trial validation | EU reference method |
| QT-EVE-ZM-031 | event-specific | DP915635 |
Quantitative PCR method for detection of maize event DP915635 (EU-RL GMFF GMOMETHODS database as of May 5, 2023). | ring trial validation | EU reference method |
| QT-EVE-GM-018 | event-specific | GMB151 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GMB151 (EU-RL GMFF GMOMETHODS database as of August 10, 2020) | ring trial validation | EU reference method |
| QT-EVE-GH-013 | event-specific | GHB811 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB811 (EU-RL GMFF GMOMETHODS database as of August 5, 2020) | ring trial validation | EU reference method |
| QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
| QT-EVE-ZM-030 | event-specific | MON87429 |
Quantitative PCR method for detection of maize event MON87429 (EU-RL GMFF GMOMETHODS database as of August 30, 2021) | ring trial validation | EU reference method |
| QT-EVE-ZM-033 | event-specific | MON95379 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON95379 (EU-RL GMFF GMOMETHODS database as of December 20, 2022) | ring trial validation | EU reference method |
| QT-EVE-GM-014 | event-specific | DAS81419 |
Quantitative PCR method for detection of soybean event DAS81419 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
| QT-EVE-GM-015 | event-specific | DAS44406 |
Quantitative PCR method for detection of soybean event DAS44406 (EURL GMFF GMOMETHODS database as of May 28, 2015) | ring trial validation | EU reference method |
| QT-EVE-OS-001 | event-specific | Golden Rice 2 |
Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
| QL-EVE-816AvaF/817AvaR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 1 – Avalon) (BVL G30.40-6). | ring trial validation | national standard |
| QL-EVE-319FalF/320FalR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 2 – Falcon) (BVL G30.40-6) | ring trial validation | national standard |
| QL-EVE-375LibF/376LibR | event-specific | Liberator |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6). | ring trial validation | national standard |
| QL-EVE-MDB110/SHA016 | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) | in-house validation | EU reference method |
| QL-EVE-SQ0718/SQ0719 | event-specific | MON71800 |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013). | in-house validation | none |
| QT-EVE-GM-017 | event-specific | SYHT0H2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event SYHT0H2(EU-RL GMFF GMOMETHODS database as of September 19, 2016) | ring trial validation | EU reference method |
| QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
| QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |
| QT-EVE-GM-013 | event-specific | DAS68416 |
Quantitative real-time PCR (TaqMan) method for the detection of soybean event DAS68416 (EU-RL GMFF GMOMETHODS database as of July 2, 2015) | ring trial validation | EU reference method |
| QT-EVE-CP-001 | event-specific | Huanong No. 1 |
Quantitative real-time (TaqMan) PCR method for detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 13, 2016). | ring trial validation | EU reference method |
| QL-EVE-CP-001 | event-specific | Huanong No. 1 |
Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016) | ring trial validation | EU reference method |
| QT-EVE-GM-010 | event-specific | MON87701 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-002 | event-specific | MON87769 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87769 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-004 | event-specific | A2704-12 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-009 | event-specific | T304-40 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event T304-40 (EU-RL GMFF GMOMETHODS database as of January 27, 2014) | ring trial validation | EU reference method |
| QL-EVE-ZM-001 | event-specific | MON810 |
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QT-EVE-GH-005 | event-specific | MON15985 |
Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-021 | event-specific | 98140 |
Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-022 | event-specific | MIR162 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-005 | event-specific | GTS 40-3-2 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-006 | event-specific | MON89788 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON89788 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-007 | event-specific | A5547-127 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-009 | event-specific | DP356043 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-001a | event-specific | 281-24-236 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 281-24-236 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-002 | event-specific | LLCotton25 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-003 | event-specific | MON1445 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON1445 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-008 | event-specific | GHB119 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB119 (EU-RL GMFF GMOMETHODS database as of July 21, 2014). | ring trial validation | EU reference method |
| QT-EVE-OS-002 | event-specific | LLRICE62 |
Quantitative real-time PCR (TaqMan) method for detection of rice event LLRICE62 (EU-RL GMFF GMOMETHODS database as of January 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-001b | event-specific | 3006-210-23 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event 3006-210-23 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-GM-008 | event-specific | DP305423 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP305423 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-004 | event-specific | MON531 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-007 | event-specific | MON88913 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON88913 (EU-RL GMFF GMOMETHODS database as January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-001 | event-specific | T45 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-002 | event-specific | Ms8 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-003 | event-specific | Rf3 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-004 | event-specific | GT73 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ST-001 | event-specific | EH92-527-1 |
Quantitative real-time PCR (TaqMan) method for detection of potato event EH92-527-1 (EU-RL GMFF GMOMETHODS database as January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-GH-006 | event-specific | GHB614 |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-008 | event-specific | NK603 |
Quantitative real-time PCR (TaqMan) method for detection of maize event NK603 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-BN-005 | event-specific | Ms1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-006 | event-specific | Rf1 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | in-house validation | EU reference method |
| QT-EVE-BN-008 | event-specific | Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | in-house validation | EU reference method |
| QT-EVE-GM-011 | event-specific | CV127 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event CV127 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) | ring trial validation | EU reference method |
| QL-EVE-OS-001 | event-specific | LLRICE601 |
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). | in-house validation | none |
| QT-EVE-GM-003 | event-specific | MON87705 |
Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87705 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-005 | event-specific | MON87460 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-006 | event-specific | Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570) | ring trial validation | ISO standard |
| QT-EVE-ZM-007 | event-specific | GA21 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-011 | event-specific | T25 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-012 | event-specific | DAS59122 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-014 | event-specific | GA21 |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-015 | event-specific | Bt11 |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-018 | event-specific | MON89034 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON89034 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) | ring trial validation | EU reference method |
| QT-EVE-ZM-020 | event-specific | MON810 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570) | ring trial validation | ISO standard |
| QT-TAX-GM-001 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene | ring trial validation | ISO standard |
| QL-TAX-SL-005 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). | ring trial validation | unknown |
| QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QT-TAX-zSSIIb-3F/zSSIIb-4R | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) | unknown | unknown |
| QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard |
| QT-TAX-SL-002 | taxon-specific | Solanum lycopersicum |
Quantitative real-time PCR (TaqMan) method for detection of the tomato LAT52 gene (ISO 21570 Amd1) | ring trial validation | ISO standard |
| QT-TAX-OS-003 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) | ring trial validation | unknown |
| QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
| QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
| QL-TAX-CMV-001 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
| QT-TAX-ZM-005 | taxon-specific | Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). | ring trial validation | EU reference method |
| QT-TAX-ZM-004 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method |
| QL-TAX-Acc1-F/Acc1-R | taxon-specific | Medicago sativa |
Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018). | ring trial validation | national standard |
| QT-TAX-ZM-008 | taxon-specific | Zea mays |
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QL-TAX-SS-AM5F/AM5R | taxon-specific | Salmo salar |
Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020) | in-house validation | unknown |
| QT-TAX-GH-020 | taxon-specific | Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-GM-003 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-BN-003 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) | ring trial validation | EU reference method |
| QT-TAX-GM-005 | taxon-specific | Glycine max |
Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023). | ring trial validation | EU reference method |
| QL-TAX-LU-001 | taxon-specific | Linum usitatissimum |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) | in-house validation | none |
| QT-TAX-OS-002 | taxon-specific | Oryza sativa |
Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016) | ring trial validation | EU reference method |
| QL-TAX-ZM-002 | taxon-specific | Zea mays |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-BN-003 | taxon-specific | Brassica napus |
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-GM-001 | taxon-specific | Glycine max |
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QT-TAX-CP-001 | taxon-specific | Carica papaya |
Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016) | ring trial validation | EU reference method |
| QT-TAX-BN-001 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method |
| QT-TAX-BN-002 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4 |
ring trial validation | EU reference method |
| QT-TAX-ST-010 | taxon-specific | Solanum tuberosum |
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) | ring trial validation | EU reference method |
| QL-TAX-CP-001 | taxon-specific | Carica papaya |
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) | ring trial validation | EU reference method |
| QL-TAX-PS-001 | taxon-specific | Pisum sativum |
Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017) | ring trial validation | EU reference method |