Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
| QL-ELE-P-SSuAra-F/P-SSuAra-R | element-specific | P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-TA29-F/P-TA29-R | element-specific | P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-Ubi-F/P-Ubi-R | element-specific | P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-nos-F/P-nos-R | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-PFMV 1-5'/PFMV 1-3' | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) | in-house validation | none |
| QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none |
| QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). | unknown | none |
| QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-OCS-F/T-OCS-R | element-specific | T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-T-g7-F/T-g7-R | element-specific | T-orf23-RHIRD, T-transcript 7 |
Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
| QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-cry3A-F/cry3A-R | element-specific | CS-cry3A-BACTT, CS-mcry3A-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) | in-house validation | national standard |