Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QT-ELE-00-002 | element-specific | CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) | ring trial validation | unknown |
| QL-TAX-SL-005 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). | ring trial validation | unknown |
| QT-EVE-BN-007 | event-specific | Rf2 |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) | in-house validation | unknown |
| QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
| QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
| QL-CON-00-015 | construct-specific | Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC) | in-house validation | none |
| QL-EVE-ZM-P1waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QL-EVE-ZM-P3waxy/P2waxy | event-specific | GE-CRISPR-Cas Waxy Corn |
Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020). | unknown | none |
| QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). | unknown | none |
| QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 | element-specific | CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). | in-house validation | none |
| QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | A1-DFR Petunias, RL01-15, RL01-17 |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QL-ELE-cat-F/R | element-specific | V-chloramphenicol acetyl transferase |
Qualitative real-time PCR (TaqMan) method for detection of the chloramphenicol marker potentially harboured by GMM in food enzyme preparations (Turgeon et al. 2008).
In term of food and feed safety, to evaluate the level of risks of likely AMR (antimicrobial resistance) gene acquisition by pathogens and gut microbiota, it is essential to determine if the full-length AMR genes are present. In order to assess the presence of the full-length cat gene a successive nested-PCR method can be performed to amplify a large fragment of the cat gene be performed (Fraiture et al. 2020) (see Related Methods QL-ELE-cat-F1/R1 and QL-ELE-cat-F2/R2). |
unknown | none |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QT-TAX-wx012-5'/wx012-3' | taxon-specific | Triticum aestivum |
Quantitative real-time PCR (TaqMan) method for detection of the common wheat waxy-D1 gene (Iida et al., 2005) | in-house validation | none |
| QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2 | element-specific | CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013). | in-house validation | none |
| QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-Vip3A-F/Vip3A-R | element-specific | CS-vip3Aa20-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013). | in-house validation | none |
| QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |