Detection methods search result
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Name |
Type |
Target | Description |
Validation |
Standardisation |
|---|---|---|---|---|---|
| QL-EVE-DC-004 | event-specific | 26407 |
Qualitative PCR method for detection of carnation event 26407 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/694) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-DC-005 | event-specific | 27531 |
Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016) | in-house validation | EU reference method |
| QL-ELE-00-006 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) | ring trial validation | national standard |
| QL-ELE-cat-F1/R1 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR for detection of the full-length Chloramphenicol marker gene that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (Turgeon, Laflamme, Ho, & Duchaine, 2008)(see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of this method and the related method QL-ELE-cat-F2/R2. |
unknown | none |
| QL-BAC-27f/926R | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from bacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of bacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QL-BAC-16S-FW/16S-Rev | taxon-specific | bacteria |
Qualitative conventional PCR for the amplification of the 16S RNA gene from eubacteria and archaebacteria.
This method is meant to enable species identification or assignment of bacterial nucleotide sequence to a taxonomical group of eubacteria and archaebacteria since the target sequence includes variable and highly variable sequences that might serve as signature sequences. Therefore the herein described PCR method should be performed and followed by sequence analysis of the amplified PCR product. (Official Collection of Methods according to § 28b GenTG, G 21.40-1). |
ring trial validation | national standard |
| QL-ELE-00-005 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-ELE-00-004 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) | ring trial validation | ISO standard |
| QL-ELE-00-001 | element-specific | P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) | ring trial validation | national standard |
| QL-ELE-00-010 | element-specific | E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) | ring trial validation | ISO standard |
| QL-TAX-DC-001 | taxon-specific | Dianthus caryophyllus |
Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016). | in-house validation | EU reference method |
| QL-EVE-DC-006 | event-specific | 123.8.8 |
Qualitative conventional PCR method for detection of carnation event 123.8.8 (EURL GMFF GMOMETHODS database as of December 15, 2017). | in-house validation | EU reference method |
| QL-PLN-00-007 | taxon-specific | all-plants |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-CON-35S-5' primer A/A1-3' primer B | construct-specific | A1-DFR Petunias, RL01-15, RL01-17 |
Qualitative conventional PCR method for detection of junction between the 35S promoter (P-35S CaMV) and the maize A1 gene (dihydroflavonol-4-reductase) of Zea mays (Meyer et al., 1993) | unknown | none |
| QL-EVE-ZM-002 | event-specific | Bt10 maize |
Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC) | in-house validation | none |
| QL-CON-00-003 | construct-specific | Bt10 maize, Bt11 |
Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-004 | construct-specific | Bt176 |
Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-005 | construct-specific | 32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-00-002 | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-00-007 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) | ring trial validation | national standard |
| QL-ELE-00-009 | element-specific | T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) | ring trial validation | ISO standard |
| QL-TAX-BN-004 | taxon-specific | Brassica napus |
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). | ring trial validation | unknown |
| QL-TAX-OS-006 | taxon-specific | Oryza sativa |
Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009). | ring trial validation | unknown |
| QL-CON-00-001 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-CON-00-006 | construct-specific | GTS 40-3-2 |
Qualitative conventional PCR method for detection of soybean event GTS 40-3-2 (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-GM-008 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-GM-002 | taxon-specific | Glycine max |
Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-TAX-CP-001 | taxon-specific | Carica papaya |
Qualitative conventional PCR method for detection of the chymopapain (chy) gene from papaya (EU-RL GMFF GMOMETHODS database as of May 23, 2016) | ring trial validation | EU reference method |
| QL-ELE-cat-F2/cat-R2 | element-specific | V-chloramphenicol acetyl transferase |
Qualitative conventional PCR method for detection of the full-length chloramphenicol marker that is frequently harboured by GMM in food enzyme preparations (Fraiture et al. 2020).
In detail, the potential presence of the chloramphenicol marker is first screened using a real-time PCR method (see Related Methods QL-ELE-cat-F/R). In case a positive real-time PCR signal is obtained, the presence of the full-length chloramphenicol marker is then evaluated by a nested-PCR consisting of the related method QL-ELE-cat-F1/cat-R1 and this method. |
unknown | none |
| QL-CON-PGS-bar-A2/PGS-bar-B2 | construct-specific | Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea) |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). | ring trial validation | national standard |
| QL-CON-CP1/pFMV2 | construct-specific | GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11). | ring trial validation | national standard |
| QL-TAX-ZM-003 | taxon-specific | Zea mays |
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) | ring trial validation | ISO standard |
| QL-ELE-RERIO 2206/RERIO 2207 | element-specific | CS-DsRed2 fluorescent protein |
Qualitative conventional PCR method for detection of the modified red fluorescent protein (DsRed2) marker gene (Rehbein & Bogerd, 2007). | unknown | none |
| QL-ELE-00-003 | element-specific | CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) | ring trial validation | national standard |
| QL-TAX-SL-005 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008). | ring trial validation | unknown |
| QL-CON-00-002 | construct-specific | Nema282F |
Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569) | ring trial validation | ISO standard |
| QL-TAX-SL-001 | taxon-specific | Solanum lycopersicum |
Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005). | ring trial validation | ISO standard |
| QL-EVE-DC-001 | event-specific | 123.2.38 |
Qualitative conventional PCR method for the detection of carnation event 123.2.38 (FLO-40644-6, previously known as FLO-40644-4) (verified by the CRL GMFF in the context of Commission Decision 2007/364/EC)(EURL GMFF GMOMETHODS database as of May 18, 2016). | in-house validation | EU reference method |
| QL-EVE-DC-003 | event-specific | 25958 |
Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016) | in-house validation | EU reference method |
| QL-EVE-ZM-001 | event-specific | MON810 |
Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569). | ring trial validation | ISO standard |
| QL-EVE-BN-002 | event-specific | GT73 |
Qualitative conventional PCR method for the detection of oilseed rape event GT73 (ISO 21569). | ring trial validation | ISO standard |
| QL-EVE-CP-001 | event-specific | Huanong No. 1 |
Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016) | ring trial validation | EU reference method |
| QL-ELE-00-019 | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-022 | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-020 | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-021 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-017 | element-specific | P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-ELE-00-023 | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). | ring trial validation | EU reference method |
| QL-ELE-00-018 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-ZM-002 | taxon-specific | Zea mays |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-BN-003 | taxon-specific | Brassica napus |
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-GM-001 | taxon-specific | Glycine max |
Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown |
| QL-TAX-LU-001 | taxon-specific | Linum usitatissimum |
Qualitative real-time PCR (TaqMan) method for detection flax stearoyl-acyl carrier protein desaturase gene of flax/linseed (EU-RL GMFF GMOMETHODS database as of January 16, 2020) | in-house validation | none |
| QL-TAX-CMV-001 | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-ELE-00-012 | element-specific | E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-00-015 | element-specific | E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). | ring trial validation | ISO standard |
| QL-TAX-CaMVF/CaMVR | taxon-specific | Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) | in-house validation | none |
| QL-ELE-PFMV 1-5'/PFMV 1-3' | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) | in-house validation | none |
| QL-ELE-P-TA29-F/P-TA29-R | element-specific | P-ta29-TOBAC |
Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013) | in-house validation | none |
| QL-ELE-P-SSuAra-F/P-SSuAra-R | element-specific | P-rbcS-ARATH |
Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013) | in-house validation | none |
| QL-EVE-558F/558R | event-specific | e871 Bacillus subtilis |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). | unknown | none |
| QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 | element-specific | CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). | in-house validation | none |
| QL-CON-00-010 | construct-specific | FP967 |
Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2) | ring trial validation | ISO standard |
| QL-EVE-MS-J101-F/J101-R | event-specific | J101 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J101 (Guertler et al. 2018) | ring trial validation | national standard |
| QL-EVE-MS-J163-F/J163-R | event-specific | J163 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-EVE-MS-KK179-F/KK179-R | event-specific | KK179 |
Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018). | ring trial validation | national standard |
| QL-TAX-AT-001 | element-specific | CS-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022). | ring trial validation | ISO standard |
| QL-ELE-00-008 | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) | ring trial validation | ISO standard |
| QL-ELE-P-nos-F/P-nos-R | element-specific | P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-ELE-00-011 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). | ring trial validation | ISO standard |
| QL-ELE-00-013 | element-specific | T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation | ISO standard |
| QL-ELE-T-OCS-F/T-OCS-R | element-specific | T-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013) | in-house validation | none |
| QL-EVE-MDB110/SHA016 | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) | in-house validation | EU reference method |
| QL-EVE-Oxy-3F/Oxy-4R | event-specific | OXY-235 |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008) | in-house validation | none |
| QL-EVE-816AvaF/817AvaR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 1 – Avalon) (BVL G30.40-6). | ring trial validation | national standard |
| QL-EVE-319FalF/320FalR | event-specific | Falcon GS 40/90 |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 2 – Falcon) (BVL G30.40-6) | ring trial validation | national standard |
| QL-EVE-375LibF/376LibR | event-specific | Liberator |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6). | ring trial validation | national standard |
| QL-TAX-FMVorf7-F4/FMVorf7-R4 | taxon-specific | Figwort mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of orf7 from figwort mosaic virus (Moor et al., 2012). | in-house validation | national standard |
| QL-EVE-16-0-1RB-F/16-0-1RB-R | event-specific | 16-0-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016) | unknown | none |
| QL-EVE-18-2-4RB-F/18-2-4RB-R | event-specific | 18-2-4 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) | unknown | none |
| QL-CON-00-012 | construct-specific | 55-1, 63-1 |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569) | ring trial validation | ISO standard |
| QL-TAX-OS-002 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). | in-house validation | unknown |
| QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none |
| QL-ELE-P-Rice actin-F/P-Rice actin-R | element-specific | P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). | in-house validation | none |
| QL-CON-00-007 | construct-specific | Huahui No.1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd) | ring trial validation | ISO standard |
| QL-EVE-OS-KM2_for/KM1_rev | event-specific | KMD1 |
Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1 | in-house validation | none |
| QL-EVE-Kef6 forward/Kef6 reverse | event-specific | Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012) | in-house validation | none |
| QL-EVE-OS-001 | event-specific | LLRICE601 |
Qualitative real-time PCR (TaqMan) method for detection of rice event LLRICE601 (verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC). | in-house validation | none |
| QL-TAX-OS-003 | taxon-specific | Oryza sativa |
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) | ring trial validation | national standard |
| QL-EVE-SS-AquAd-F/AquAd-R | event-specific | AquAdvantage Salmon |
Qualitative real-time PCR (TaqMan) method for detection of salmon event AquAdvantage (Soga et al., 2020) | in-house validation | national standard |
| QL-ELE-T-35S-F/T-35S-R | element-specific | T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) | in-house validation | none |
| QL-ELE-epsps2 1-5'/epsps2 1-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) | in-house validation | none |
| QL-ELE-epsps 1-5'/epsps 3-3' | element-specific | CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-P-FMV-F/P-FMV-R | element-specific | P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) | in-house validation | none |
| QL-ELE-AgroBS1 F/AgroBS1 R | element-specific | V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard |
| QL-ELE-cry1A.105 - F1/cry1A.105 - R1 | element-specific | CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-cry1A 4-5'/cry1A 4-3' | element-specific | CS-cry1Ab-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). | in-house validation | none |
| QL-ELE-cry2Ab2 - F/cry2Ab2 - R | element-specific | CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). | in-house validation | none |
| QL-ELE-bar 2-5'/bar 2-3' | element-specific | CS-bar-STRHY |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) | in-house validation | none |