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  • Citation:

    Yang L., Yang Y., Jin W., Zhang X., Li X., Wu Y., Li J., Li L. (2018) Development and interlaboratories validation of event-specific quantitative real-time PCR method for genetically modified rice G6H1 event. Journal of Agricultural and Food Chemistry, 66(30),8179-8186

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 peer-reviewed article

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Content type:
detection

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Publication Year:

 2018

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  • Abstract:

    The transgenic rice G6H1 was a new event with the traits of herbicide-tolerance and insect-resistant. Herein, we developed one event-specific real-time PCR method with high specificity and sensitivity for G6H1 event quantitative analysis, and validated its performance on practical samples quantification through a collaborative ring trial. A total of eight laboratories participated in this validation and quantified three blind G6H1 powder samples including DNA extraction and real-time PCR analysis. The statistically analyzed results from returned data confirmed its high PCR efficiency and good linearity, trueness, and precision, indicating that the developed G6H1 real-time PCR assay was accurate, reliable, and comparable for G6H1 identification and quantification.