detail view literature GMO literature details Help icon

  • Citation:

    Shindo, Y. , Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J. , Akiyama, H. , Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126

Publication type:

 peer-reviewed article

Comment:

 -

Content type:
detection

Keyword:
soybeans / maize / molecular genetics / dna

Publication Year:

 2002

Authority:

 AOAC

View PDF:

View Url:

 peer-reviewed articleLink to the document url.

  • Abstract:

    Novel analytical methods based on real-time quantitative polymerase chain reactions by use of new reference molecules were validated in interlaboratory studies for the quantitation of genetically modified (GM) maize and soya. More than 13 laboratories from Japan, Korea and the USA participated in the studies. The interlaboratory studies included 2 separate stages: (1) measurement tests of coefficient values, the ratio of recombinant DNA (r-DNA) sequence, and endogenous DNA sequence in the seeds of GM maize and GM soya; and (2) blind tests with 6 pairs of maize and soya samples, including different levels of GM maize or GM soya. Test results showed that the methods are applicable to the specific quantitation of the 5 lines of GM maize and one line of GM soya. After statistical treatment to remove outliers, the repeatability and reproducibility of these methods at a level of 5.0% were < 13.7 and 15.9%, respectively. The quantitation limits of the methods were 0.50% for Bt11, T25 and MON810, and 0.10% for GA21, Event176, and Roundup Ready soya. The results of blind tests showed that the numerical information obtained from these methods will contribute to practical analyses for labelling systems of GM crops.