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  • Citation:

    Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25.

Publication type:

 peer-reviewed article

Comment:

 180306 LH: Standardized citation to APA style. Added 2 keywords.

Content type:
detection

Keyword:
evaluation method, Protocol for PCR method

Publication Year:

 2016

Authority:

 Research group

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 peer-reviewed articleLink to the document url.

  • Abstract:

    For transferring the event-specific PCR methods of genetically modified papaya Huanong No.1 to other laboratories, we validated the previous developed PCR assays of Huanong No. 1 according to the international standard organization (ISO) guidelines. A total of 11 laboratories participated and returned their test results in this trial. In qualitative PCR assay, the high specificity and limit of detection as low as 0.1% was confirmed. For the quantitative PCR assay, the limit of quantification was as low as 25 copies. The quantitative biases among ten blind samples were within the range between 0.21% and 10.04%. Furthermore, the measurement uncertainty of the quantitative PCR results was calculated within the range between 0.28% and 2.92% for these ten samples. All results demonstrated that the Huanong No. 1 qualitative and quantitative PCR assays were creditable and applicable for identification and quantification of GM papaya Huanong No. 1 in further routine lab analysis.