GMO Detection Methods
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Alias:
Cibus Canola Event 5715, GE canola, GE rape, gene editing, Herbicide tolerant canola -
UID:
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Tradename:
SU Canola -
Companies/Institutions:
Cibus US -
Species:
Brassica napus(canola, oilseed rape, rapeseed) -
Traits:
Herbicide tolerance > ALS/AHAS inhibitor tolerance > Imidazolinone toleranceHerbicide tolerance > ALS/AHAS inhibitor tolerance > Sulfonylurea tolerance
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EU authorisation:
Name |
Type |
Target | Description |
Validation |
Standardisation |
Verification |
|---|---|---|---|---|---|---|
| QL-TAX-BN-004 | taxon-specific | Brassica napus |
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). | ring trial validation | unknown | 1 |
| QT-TAX-FatA primer1/FatA primer2 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
in-house validation | none | 1 |
| QT-TAX-BN-003 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) | ring trial validation | EU reference method | 1 |
| QL-TAX-BN-003 | taxon-specific | Brassica napus |
Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014) | ring trial validation | unknown | 1 |
| QT-TAX-BN-012 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method | 1 |
| QT-TAX-BN-001 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014)
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method | 1 |
| QT-TAX-BN-002 | taxon-specific | Brassica napus |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4 |
ring trial validation | EU reference method | 1 |