GMO Detection Methods
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Alias:
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UID:
MON-87419-8 -
Tradename:
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Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company) -
Species:
Zea mays(maize, corn) -
Traits:
Herbicide tolerance > Dicamba (2-methoxy-3,6-dichlorobenzoic acid) toleranceHerbicide tolerance > Glufosinate tolerance
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EU authorisation:
Name |
Type |
Target | Description |
Validation |
Standardisation |
Verification |
|---|---|---|---|---|---|---|
| QT-TAX-zSSIIb-3F/zSSIIb-4R | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) | unknown | unknown | 1 |
| QT-TAX-ZM-014 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-011 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) | ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-008 | taxon-specific | Zea mays |
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-006 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) | ring trial validation | ISO standard | 1 |
| QT-TAX-ZM-005 | taxon-specific | Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). | ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-004 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-003 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) | ring trial validation | EU reference method | 1 |
| QT-TAX-ZM-002 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard | 1 |
| QT-TAX-ZM-001 | taxon-specific | Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation | ISO standard | 1 |
| QT-EVE-ZM-029 | event-specific | MON87419 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON87419 (EU-RL GMFF GMOMETHODS database as of September 23, 2019) | ring trial validation | EU reference method | 3 |
| QT-ELE-00-002 | element-specific | CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) | ring trial validation | unknown | 2 |
| QL-TAX-ZM-003 | taxon-specific | Zea mays |
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) | ring trial validation | ISO standard | 1 |
| QL-TAX-ZM-002 | taxon-specific | Zea mays |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) | ring trial validation | unknown | 1 |
| QL-ELE-00-025 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. | ring trial validation | EU reference method | 3 |
| QL-ELE-00-021 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) | ring trial validation | unknown | 2 |
| QL-ELE-Patf-n2/Patr-n2 | element-specific | CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) | in-house validation | none | 3 |
| QL-ELE-AINT 2-5'/AINT 2-3' | element-specific | I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). | in-house validation | none | 3 |
| QL-ELE-AgroBS2 F/AgroBS2 R | element-specific | V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation | national standard | 2 |