detail view molecular characterisation GMO Detection Methods

  • GMO:

    MON87419
  • Alias:

  • UID:

    MON-87419-8
  • Tradename:

  • Companies/Institutions:

    Bayer Crop Science (formerly Monsanto Company)
  • Species:

    Zea mays
    (maize, corn)
  • Traits:

      Herbicide tolerance > Dicamba (2-methoxy-3,6-dichlorobenzoic acid) tolerance

      Herbicide tolerance > Glufosinate tolerance

  • EU authorisation:

    approvedapprovednot approvedapproved
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NameSort Icon TypeSort Icon Target DescriptionSort Icon ValidationSort Icon StandardisationSort Icon Verification
QL-TAX-ZM-003 taxon-specific
Zea mays
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) ring trial validation ISO standard 1
QL-ELE-00-021 element-specific
CS-pat-STRVR
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) ring trial validation unknown 2
QL-TAX-ZM-002 taxon-specific
Zea mays
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) ring trial validation unknown 1
QL-ELE-AINT 2-5'/AINT 2-3' element-specific
I-actin
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). in-house validation none 3
QL-ELE-AgroBS2 F/AgroBS2 R element-specific
V-Flanking Ti-ocs-RHIRD
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.

Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)

ring trial validation national standard 2
QL-ELE-Patf-n2/Patr-n2 element-specific
CS-pat-STRVR
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) in-house validation none 3
QL-ELE-00-025 element-specific
CS-pat-STRVR
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. ring trial validation EU reference method 3
QT-TAX-ZM-005 taxon-specific
Zea mays
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). ring trial validation EU reference method 1
QT-TAX-ZM-008 taxon-specific
Zea mays
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation EU reference method 1
QT-TAX-ZM-004 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

ring trial validation EU reference method 1
QT-TAX-ZM-003 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) ring trial validation EU reference method 1
QT-TAX-ZM-001 taxon-specific
Zea mays
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1

ring trial validation ISO standard 1
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